Molecular Stability of Clover Proliferation Phytoplasma DNA in Periwinkle Plant Tissues after Thermal Treatment under Microwave Conditions.
スポンサーリンク
概要
- 論文の詳細を見る
This paper describes a simple, rapid and economical method to preserve healthy plant tissues or those infected with phytoplasma for molecular diagnosis and analysis. After thermal treatments, clover proliferation (CP) phytoplasma DNA in periwinkle leaf samples, which was exposed to the microwave heat up to 480sec, remained intact but not after 600sec. The extracted DNA of the CP-infected tissues was amplified with phytoplasma universal primers showing a typical single band of 558bp in the microwave treated samples except the 600sec treatment, or healthy controls. Restriction digestions of PCR products of the heat treated samples with Alu I enzyme showed patterns similar to that with the digested PCR product of a fresh CP-infected sample on 5% polyacrylamide gel-electrophoresis, indicating its stability after the microwave heat treatment. The identity of CP phytoplasma in each heat-treated sample was confirmed by its amplification with specific primers forming a prominent band of 196bp which matched the size of the primer pair that was specific to CP phytoplasma.
- 日本学士院の論文
著者
-
HIRUKI Chuji
Department of Agricultural, Food and Nutritional Science, University of Alberta
-
KHADHAIR Abdul-Hameed
Department of Agricultural, Food and Nutritional Science, University of Alberta
-
MOMANI Fouad
Department of Agricultural, Food and Nutritional Science, University of Alberta
関連論文
- Genetic Differentiation of Phytoplasma Isolates Determined by a DNA Heteroduplex Mobility Assay
- Rapid detection of a single-base-pair mutation in phytoplasmal conserved genes by DNA heteroduplex mobility assay (HMA)
- Biolistic Delivery of Foreign DNA or Genomic Transcripts of Plant Virus Full-length cDNA Clones into Monocotyledonous and Dicotyledonous Plant Tissues
- Molecular evolution of phytoplasmas based on polymorphisms in the 16S rRNA genes and the 16/23S spacer regions
- Direct Sequencing of Butyrivibrio fibrisolvens 16S rRNA Genes and their 3'- and 5'-Flanking Regions.
- Viral Genome Delivery into Detached and Intact Leaf Tissues of Vigna unguiculata by RNA-coated Gold Particles Using the Improved Particle Gun.
- Molecular Stability of Clover Proliferation Phytoplasma DNA in Periwinkle Plant Tissues after Thermal Treatment under Microwave Conditions.
- PCR (Polymerase Chain Reaction)-based Selection of Phytoplasma-free Clones of Paulownia Tissue Culture after Heat Treatment of Witches'-broom.
- Distribution of Plant Pathogenic Mollicutes in Catharanthus roseus Determined by in situ Molecular Hybridization and DNA Staining with DAPI.
- Chitinase Purified from Alfalfa Infected by Clover Proliferation Mycoplasmalike Organism.
- A comparative study of the sensitivity of dot hybridization assays using cRNA and cDNA for the detection of potato spindle tuber viroid.
- Transmission of sweet clover necrotic mosaic virus.
- The use of cloned DNA probes for diagnosis of noncultivable plant mollicutes.
- DNA Amplifcation Based on Polymerase Chain Reaction for the Sensitive Detection of the Mycoplasmalike Organism Associated with Paulownia Witches'-Broom.