Quantitative Study of Anomeric Forms of Maltose Produced by α- and β-Amylases
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概要
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Anomeric forms of glucose and maltose produced from phenyl, p-nitrophenyl, p-tert-butylphenyl, p-ethylphenyl and p-chlorophenyl α-maltosides and maltopentaose by α- and β-amylases were determined quantitatively by a gas-liquid chromatographic method. All of the three kinds of α-amylases tested, B. subtilis saccharifying α-amylase, Taka-amylase A, and porcine pancreas α-amylase, were found to produce only α-maltose from the maltosides. Sweet potato and barley β-amylases produced β-maltose from maltopentaose. Saccharifying α-amylase from B. subtilis also released α-maltose from all the maltosides mentioned above, contrary to the report by Shibaoka et al. that the enzyme released β-maltose from maltosides other than phenyl α-maltoside: FEBS Lett., 16, 33 (1971); J. Biochem., 77, 1215 (1975). It appears unlikely that the a-amylase releases β-maltose, depending on the kind of substrate.
- 社団法人 日本農芸化学会の論文
著者
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Chiba Seiya
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Kimura Atsuo
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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