Quantitative Study of Anomeric Forms of Glucose Produced by α-Glucosidases and Glucoamylases
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概要
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A gas-liquid chromatographic method was applied to the determination of anomeric forms of sugar produced by carbohydrases. Anomeric forms of glucose released from maltotriose, phenyl amaltoside and phenyl a-glucoside were determined quantitatively. Thirteen a-glucosidases tested, including a-glucosidase from honey bee and acid α-glucosidase from pigs liver, were found to produce a-glucose exclusively, and two kinds of glucoamylases, only β-glucose. This method proved very useful for the determination of the anomeric forms of sugar produced. It was confirmed that mammalian acid a-glucosidase does not belong to the category of exo-1, 4-α-glucosidase (EC 3.2.1.3).
- 社団法人 日本農芸化学会の論文
著者
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Chiba Seiya
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Kimura Atsuo
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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MATSUI Hirokazu
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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