Comparison between Two Neutral α-Glucosidases from Pigs Liver and Serum
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概要
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The enzymatic, physicochemical and immunochemical properties of pig liver neutral α-glucosidase were compared with those of pig serum neutral α-glucosidase. The enzyme purified from pigs liver showed a pH-optimum of 6.5 and was stable in the range of pH 5.0 to 9.0. This enzymealso showed a temperature-optimum of 60°C and was stable up to 60°C. These properties of pH and temperature of the pig liver a-glucosidase were very similar to those of the pig serum α-glucosidase reported previously. The isoelectric points and the molecular weights of the two α-glucosidases were same: pi, 4.2 and MW, 2.7-3.0 x 105. The Km values for maltose, maltotriose and phenyl a-maltoside of both α-glucosidases were 8.5 mM, 2.2mM and 2.3 mM, respectively. The essential ionizable groups of the enzymes were identified to be a carboxyl group (-COO-) in the acidic side and an imidazole group (NH+) in the alkaline side. The pig liver a-glucosidase produced a clear precipitin line with the antiserum to the pig serum a-glucosidase, but a difference was found in their mobilities on crossed immunoelectrophoresis.
- 社団法人 日本農芸化学会の論文
著者
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Chiba Seiya
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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MATSUI Hirokazu
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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YAMADA Toshikazu
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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SOMEYA Yoshiaki
Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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