Expression of Allene Oxide Cyclase from Pharbitis nil upon Theobroxide Treatment
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概要
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In previous reports we have reported that theobroxide induces characteristic accumulation of allene oxide cyclase (AOC; EC 5.3.99.6) protein and jasmonic acid (JA) in Pharbitis nil. In the present study, PnAOC, an AOC gene from Pharbitis nil was cloned. Immunofluorescence assays indicated that the AOC protein is located in the chloroplast of vascular bundles in Pharbitis nil leaves. The PnAOC cDNA sequence lacking the chloroplast signal peptide was successfully expressed in Escherichia coli, and a gas chromatography-mass spectrum assay suggested the relative AOC activity of the recombinant PnAOC protein in comparison with Arabidopsis AOC2. Interestingly, a biphasic expression of PnAOC was induced by theobroxide, which is consistent with the accumulation patterns of AOC protein and JA. All these results indicate that AOC is the primary target of theobroxide regulation and suggest that feedback regulation of PnAOC by JA occurs upon theobroxide treatment in Pharbitis nil.
- 社団法人 日本農芸化学会の論文
著者
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TAKAHASHI Kosaku
Research Institute of Life Science, Snow Brand Milk Products Co.,Ltd.
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OTAGAKI Shungo
Graduate School of Agriculture, Hokkaido University
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KONG Fan-Jiang
Research Faculty of Agriculture, Hokkaido University
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LI Ying
Graduate School of Agriculture, Hokkaido University
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ABE Jun
Research Faculty of Agriculture, Hokkaido University
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LIU Baohui
Northeast Insititute of Geography and Agroecology, Chinese Academy of Sciences
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SCHALLER Florian
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum
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PIOTROWSKI Markus
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum
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MATSUURA Hideyuki
Research Faculty of Agriculture, Hokkaido University
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YOSHIHARA Teruhiko
Research Faculty of Agriculture, Hokkaido University
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NABETA Kensuke
Research Faculty of Agriculture, Hokkaido University
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