Effects of Air Flow on Production of Vaccine Protein against Swine Edema Disease in Transgenic Lettuce

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概要

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• The transgenic lettuce expressing double repeated Stx2eB (2×Stx2eB) is considered to be an edible vaccine candidate against swine edema disease. For the efficient vaccine protein production in transgenic lettuce grown in a plant factory, the effects of air flow on vaccine protein production in transgenic lettuce were investigated. Plants were grown hydroponically in a closed-type plant factory for 21 days with four airflow patterns: 1) air flow from the side to leaves at 2.0 m s<SUP>−1</SUP>; 2) air flow from the top to the inner developing leaves with flexible transparent tubing at 1.0 m s<SUP>−1</SUP>; 3) air flow from the bottom to leaves with transparent tubing at 1.0 m s<SUP>−1</SUP>; and 4) no air flow. Our results showed that the total leaf number in air flow from the top and the bottom to leaves was significantly increased compared with that in no air flow. While lettuce growth with inner air flow and bottom air flow was enhanced, tipburn at the developing leaves were eliminated by air flow. 2×Stx2eB per plant in bottom air flow to leaves was the highest of the four air flow patterns. These results suggest that bottom air flow to the leaves can enhance vaccine protein productivity in transgenic lettuce.

著者

• Kato Ko

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Kato Ko

  Graduate School Of Biological Science Nara Institute Of Science And Technology

• Takayama Kotaro

  Faculty Of Agriculture Ehime University

• Sawada Kazutoshi

  Advanced Technology Research Laboratories Idemitsu Kosan Co. Ltd.

• Nishina Hiroshige

  Faculty Of Agriculture Ehime University

• Murase Haruhiko

  Graduate School Of Agriculture And Biological Science Osaka Prefecture University

• Matsui Takeshi

  Advanced Technology Research Laboratories Idemitsu Kosan Co. Ltd.

• OKAMURA Ken-Ichi

  R&D. Division, NISSHOKU Group Inc.

• MATSUDA Yoshie

  R&D. Division, NISSHOKU Group Inc.

• IGARI Kadunari

  R&D. Division, NISSHOKU Group Inc.

• ASAO Hiroshi

  Production Technic Division, Nara Prefectural Agricultural Experiment Station

• Asao Hiroshi

  Production Technic Division Nara Prefectural Agricultural Experiment Station

• Takita Eiji

  Advanced Technology Research Laboratories Idemitsu Kosan Co. Ltd.

• Takahashi Noriko

  Faculty Of Agriculture Ehime University

• OKAMURA Ken-Ichi

  R&D. Division, NISSHOKU Group Inc

• MATSUDA Yoshie

  R&D. Division, NISSHOKU Group Inc

• IGARI Kadunari

  R&D. Division, NISSHOKU Group Inc

• MATSUI Takeshi

  Advanced Technology Research Laboratory

• MURASE Haruhiko

  Graduate School of Engineering, Osaka Prefecture University

• TAKITA Eiji

  Advanced Technology Research Laboratory

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