A Dynamic Model for Simulating Recombinant Protein Production in Transgenic Lettuce for Optimum Environmental Control
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概要
- 論文の詳細を見る
A dynamic model for simulating recombinant protein production in transgenic lettuce for optimum environmental control was developed. The model comprised three main parameters, which were leaf fresh weight, the number of cells, and amount of protein in each leaf. The differential equations of the model were integrated using the modeling software STELLATM. The model was calibrated based on the measured data of transgenic lettuce plants which were grown in a plant factory for 50 days from seeding. The plants contained an introduced Cauliflower mosaic virus-35S promoter fused onto the β-glucuronidase gene. Simulations were conducted to investigate the effect of plant growth control on the productivity of recombinant proteins which have various stabilities. The simulations indicated the importance of recombinant protein stability. If the parameters corresponding to the production and degradation of a recombinant protein can be identified, the simulation will be a strong tool for devising an environmental control strategy for highly efficient recombinant protein production systems.
- 日本生物環境工学会の論文
- 2009-09-30
著者
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金井 猛徳
大阪府立大学大学院生命環境科学研究科
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Kanai Takenori
Osaka Prefecture Univ. Osaka Jpn
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Kanai Takenori
Graduate School Of Life And Environmental Science Osaka Prefecture University
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Kanai Takenori
Laboratory Of Environmental Information Science And Application Engineering Graduate School Of Agric
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OKAYAMA Tsuyoshi
Osaka Branch, Okayama Laboratory, NISSHOKU Corporation
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OKAMURA Kenichi
Osaka Branch, Okayama Laboratory, NISSHOKU Corporation
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MURASE Haruhiko
Graduate School of Life and Environmental Science, Osaka Prefecture University
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Murase Haruhiko
Graduate School Of Life And Environmental Science Osaka Prefecture University
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Murase Haruhiko
Graduate School Of Life And Environmental Sciences Osaka Prefecture Univ.
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Okayama Tsuyoshi
Department Of Food Agricultural And Biological Engineering
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USHADA Mirwan
Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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Okamura Kenichi
Osaka Branch Okayama Laboratory Nisshoku Corporation
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Ushada Mirwan
Osaka Prefecture Univ. Osaka Jpn
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Okayama Tsuyoshi
Osaka Branch Okayama Laboratory Nisshoku Corporation
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Ushada Mirwan
Graduate School Of Life And Environmental Science Osaka Prefecture University
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Murase Haruhiko
Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University
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Murase Haruhiko
Graduate School Of Agriculture And Biological Science Osaka Prefecture University
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