An Effective Method of Screening Glucose-rich Microbial Culture Filtrates for Insect Trehalase Inhibitors
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概要
- 論文の詳細を見る
Trehalase inhibitors are promising as a specific insect growth regulator. Microbial culture filtrates are a good source of bioactive compounds, but some of them retain a significant amount of glucose, a major component of the culture medium, which makes it difficult to detect trehalase inhibitors because of high blank values for the glucose determination. To find a variety of trehalase inhibitors in glucose-rich microbial culture filtrates, an effective method for removing glucose by enzymatic conversion prior to a routine trehalase-inhibitory assay was devised. Conversion with glucose oxidase and catalase could completely remove 1Omg/ml of glucose, and a wide variety of microbial culture filtrates with high glucose content could then be subjected to the routine bioassay. This method can also be applied to screen inhibitors for other enzymes whose activity is assayed by the amount of glucose formed.
- 社団法人日本農芸化学会の論文
- 1995-03-23
著者
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KAWABE Akira
Department of Biotechnology, Graduate School of Engineering, Osaka University
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神崎 浩
岡山大 大学院自然科学研究科(農学系)
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KANZAKI HIROSHI
Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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KAWAZU KAZUYOSHI
Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Kobayashi A
Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Kobayashi Akio
Laboratory of Food Chemistry, Department of Nutrition and Food Science, Ochanomizu University
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Kanzaki Hiroshi
Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Kawazu Kazuyoshi
The Graduate School Of Natural Sci. And Technol. Dep. Of Biofunctional Chemistry Lab. Of Applied Nat
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Kawazu Kazuyoshi
Laboratory Of Bioresources Chemistry Department Of Agricutlural Science Okayama University
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Kawazu Kazuyoshi
Department Of Bioresources Chemistry Okayama University
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Kawazu Kazuyoshi
Laboratory Of Bioresources Chemistry Department Of Agricultural Science Okayama University
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Niihama Takafumi
Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Kaji Hiroaki
Department Of Immunochemistry Faculty Of Pharmaceutical Sciences Okayama University
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Kawazu K
The Graduate School Of Natural Sci. And Technol. Dep. Of Biofunctional Chemistry Lab. Of Applied Nat
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Niihama Takafumi
Laboratory Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Kobayashi Akio
Laboratory Of Bioresources Chemistry Department Of Agricultural Science Okayama University
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