Improved Bile Acid-binding Ability of Soybean Glycinin A1a Polypeptide by the Introduction of a Bile Acid-binding Peptide (VAWWMY)

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概要

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• We have previously identified a potential bile acid-binding peptide sequence (VAWWMY) in acidic polypeptide A1a of the soybean glycinin A1aB1b subunit (Choi, S. K., et al., Biosci. Biotechnol. Biochem., 66, 2395–2401 (2002)). In this study, we introduced the nucleotide sequence encoding this peptide in the coding DNA which corresponds to amino acids between 251 and 256, and 282 and 287 into the A1a polypeptide by replacement to respectively give modified versions A1aM1 and A1aM2. A fluorescence analysis demonstrates that their bile acid-binding ability was improved compared to A1a. Moreover, modified proglycinin A1aB1b with the VAWWMY sequence at the same sites as those of A1aM1 and A1aM2 was judged to assume the correct conformation. These results suggest the possibility of developing transgenic crops to accumulate the modified glycinin.

• 社団法人 日本農芸化学会の論文
• 2004-09-23

著者

• Choi Seon-kang

  Laboratory Of Food Quality Design And Development Graduate School Of Agriculture Kyoto University

• ADACHI Motoyasu

  Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University

• UTSUMI Shigeru

  Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University

• Adachi Motoyasu

  Laboratory Of Food Quality Design And Development Graduate School Of Agriculture Kyoto University

• Choi Seon-kang

  Laboratory Of Applied Molecular Design Marine Biotechnology Institute

• Utsumi S

  Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University

• Adachi M

  Laboratory Of Food Quality Design And Development Graduate School Of Agriculture Kyoto University

• Utsumi Shigeru

  Laboratory Of Food Quality Design And Development Division Of Agronomy And Horticultural Science Gra

関連論文

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• Improved Bile Acid-binding Ability of Soybean Glycinin A1a Polypeptide by the Introduction of a Bile Acid-binding Peptide (VAWWMY)
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