High-level Expression, Purification and Functional Properties of Soybean Proglycinin from Escherichia coli(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
The proglycinin synthesized in E. coli JM105 comprised approximately 20 % of the total bacterial proteins, with a yield of 39 mg per liter of culture under the optimum cultivation conditions. The proglycinin was purified to homogeneity by salt precipitation, ion-exchange chromatography, and cryoprecipitation. The purified proglycinin self-assembled to a trimer with a secondary structure similar to that of the glycinin half-molecule from soybean seeds, and had properties of gel formation by heating and precipitation with calcium salt as the native glycinin and glycinin half-molecule do. This indicated that the E. coli expression system of glycinin cDNA may be used for the evaluation of the self-assembly and the food qualities of protein-engineered soybean proteins.
- 社団法人日本農芸化学会の論文
- 1990-06-23
著者
-
Kim Chan-shick
The Research Institute For Food Science Kyoto University
-
Utsumi S
Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University
-
Utsumi Shigeru
The Research Institute For Food Science Kyoto University
-
Kamiya Seigo
The Research Institute For Food Science Kyoto University
-
Kohno M
Food Science Research Institute Fuji Oil Co. Ltd.
-
KANAMORI Jiro
The Research Institute for Food Science, Kyoto University
-
KITO Makoto
The Research Institute for Food Science, Kyoto University
-
Kanamori Jiro
The Research Institute For Food Science Kyoto University
関連論文
- Soybean Glycinin A1aB1b Subunit Has a Molecular Chaperone-like Function to Assist Folding of the Other Subunit Having Low Folding Ability
- Improved Bile Acid-binding Ability of Soybean Glycinin A1a Polypeptide by the Introduction of a Bile Acid-binding Peptide (VAWWMY)
- Identification of the Bile Acid-binding Region in the Soy Glycinin A1aB1b Subunit(Food & Nutrition Science)
- Safety Assessment of Rice Genetically Modified with Soybean Glycinin by Feeding Studies on Rats
- Quality and Safety Evaluation of Genetically Engineered Rice with Soybean Glycinin : Analyses of the Grain Composition and Digestibility of Glycinin in Transgenic Rice
- The Crystal Structure of Lipase II from Rhizopus niveus at 2.2 Å Resolution
- Purification, Characterization, and Crystallization of Two Types of Lipase from Rhizopus niveus
- Safety Assessment of Genetically Engineered Food : Detection and Monitoring of Glyphosate-Tolerant Soybeans
- Interactions During Heat-induced Gelation in a Mixed System of Soybean 7S and 11S Globulins(Food & Nutrition)
- Mechanism of Heat-Induced Gelation and Gel Properties of Soybean 7S Globulin(Food & Nutrition)
- Crystallization and Preliminary X-Ray Analysis of Soybean Proglycinins Modified by Protein Engineering
- Safety Assessment of Transgenic Potatoes with Soybean Glycinin by Feeding Studies in Rats
- Soybean β-Conglycinin Diet Suppresses Serum Triglyceride Levels in Normal and Genetically Obese Mice by Induction of β-Oxidation, Downregulation of Fatty Acid Synthase, and Inhibition of Triglyceride Absorption
- Introduction of DPR, an Enterostatin Fragment Peptide, into Soybean β-Conglycinin α' Subunit by Site-directed Mutagenesis
- Functions of Characteristic Cys-Gly-His-Cys (CGHC) and Gln-Glu-Asp-Leu (QEDL) Motifs of Microsomal ER-60 Protease
- Gelling Properties of Soybean β-Conglycinin Having Different Subunit Compositions
- Introduction of a Low Molecular Weight Agonist Peptide for Complement C3a Receptor into Soybean Proglycinin A_B_ Subunit by Site-directed Mutagenesis
- Introduction of Enterostatin (VPDPR) and a Related Sequence into Soybean Proglycinin A_B_ Subunit by Site-directed Mutagenesis
- Amino Acid Sequence Analysis of Bitter Peptides from a Soybean Proglycinin Subunit Synthesized in Escherichia coli
- Reduction by Phytate-reduced Soybean β-Conglycinin of Plasma Triglyceride Level of Young and Adult Rats
- Cloning and Sequence Analysis of cDNA encoding Rhizopus niveus Lipase
- Decreases in Serum Triacylglycerol and Visceral Fat Mediated by Dietary Soybean β-conglycinin
- Effects of Soybean β-Conglycinin on Hepatic Lipid Metabolism and Fecal Lipid Excretion in Normal Adult Rats
- Effects of Soybean β-Conglycinin on Body Fat Ratio and Serum Lipid Levels in Healthy Volunteers of Female University Students
- Novel Method Using Phytase for Separating Soybean β-Conglycinin and Glycinin
- High-level Expression, Purification and Functional Properties of Soybean Proglycinin from Escherichia coli(Biological Chemistry)
- Polymorphism and Expression of cDNAs Encoding Glycinin Subunits(Food & Nutrition)
- Modification Tolerability of Soybean Proglycinin