Introduction of DPR, an Enterostatin Fragment Peptide, into Soybean β-Conglycinin α' Subunit by Site-directed Mutagenesis
スポンサーリンク
概要
- 論文の詳細を見る
DPR, a fragment peptide of enterostatin (VPDPR) having hypocholesterolemic activity, was introduced into the three homologous sites, EPR, DYR, and DPI, in the soybean β-conglycinin α′ subunit by site-directed mutagenesis. The modified β-conglycinin was expressed in Escherichia coli and recovered in the soluble fraction. After purification on ion-exchange HPLC, the modified β-conglycinin was digested by trypsin to release integrated DPR. The yield of DPR from 1 mole of the modified β-conglycinin was 1.2 mole.
- 社団法人 日本農芸化学会の論文
- 2004-01-23
著者
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YOSHIKAWA Masaaki
Division of Food Bioscience and Biotechnology, Graduate School of Agriculture, Kyoto University
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YOSHIKAWA Masaaki
Graduate School of Agriculture, Kyoto University
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TAKENAKA Yasuyuki
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Utsumi S
Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University
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Utsumi Shigeru
Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University
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Maruyama N
Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University
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Maruyama Nobuyuki
Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University
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DOYAMA Naomi
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Takenaka Y
Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.
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Takenaka Yasuyuki
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Doyama Naomi
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Yoshikawa Masaaki
Kyoto Univ. Kyoto Jpn
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Takenaka Y
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Yoshikawa Masaaki
Division Of Food Bioscience And Biotechnology Graduate School Of Agriculture Kyoto University
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Yoshikawa M
Graduate School Of Agriculture Kyoto University
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Takenaka Yasuyuki
Kobe Shoin Woman's University
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