Introduction of a Low Molecular Weight Agonist Peptide for Complement C3a Receptor into Soybean Proglycinin A_<1a>B_<1b> Subunit by Site-directed Mutagenesis
スポンサーリンク
概要
- 論文の詳細を見る
LPLPR, a complement C3a agonist peptide, with hypocholesterolemic activity was introduced into the homologous site of soybean proglycinin A_<1a>B_<1b> subunit by site-directed mutagenesis. This modified proglycinin was expressed in E.coli and recovered from the insoluble fraction. LPLPR was released by the action of chymotrypsin and trypsin as expected. Furthermore, two peptides (RPSYLPLPR and PSYLPLPR) with extended sequence at the amino-terminus of LPLPR were obtained. Their ileum-contracting activity was 9〜13 times stronger than that of LPLPR. The overall yields of purified LPLPR, RPSYLPLPR and PSYLPLPR were 25%, 12%, and 0.7% respectively.
- 社団法人日本農芸化学会の論文
- 2001-05-23
著者
-
YOSHIKAWA Masaaki
Division of Food Bioscience and Biotechnology, Graduate School of Agriculture, Kyoto University
-
YOSHIKAWA Masaaki
Graduate School of Agriculture, Kyoto University
-
TAKENAKA Yasuyuki
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
-
Utsumi S
Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University
-
Utsumi Shigeru
Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University
-
Takenaka Y
Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.
-
Takenaka Yasuyuki
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
-
Yoshikawa Masaaki
Kyoto Univ. Kyoto Jpn
-
Takenaka Y
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
-
Yoshikawa Masaaki
Division Of Food Bioscience And Biotechnology Graduate School Of Agriculture Kyoto University
-
Yoshikawa M
Graduate School Of Agriculture Kyoto University
-
Takenaka Yasuyuki
Kobe Shoin Woman's University
関連論文
- Neurite Outgrowth-stimulating Activities of β-Casomorphins in Neuro-2a Mouse Neuroblastoma Cells(Food & Nutrition Sience)
- Soybean Glycinin A1aB1b Subunit Has a Molecular Chaperone-like Function to Assist Folding of the Other Subunit Having Low Folding Ability
- Improved Bile Acid-binding Ability of Soybean Glycinin A1a Polypeptide by the Introduction of a Bile Acid-binding Peptide (VAWWMY)
- Identification of the Bile Acid-binding Region in the Soy Glycinin A1aB1b Subunit(Food & Nutrition Science)
- Safety Assessment of Rice Genetically Modified with Soybean Glycinin by Feeding Studies on Rats
- Quality and Safety Evaluation of Genetically Engineered Rice with Soybean Glycinin : Analyses of the Grain Composition and Digestibility of Glycinin in Transgenic Rice
- A Potent Hypotensive Peptide, Novokinin, Induces Relaxation by AT2- and IP-Receptor-Dependent Mechanism in the Mesenteric Artery from SHRs
- Design of a Highly Potent Anti-hypertensive Peptide Based on Ovokinin(2-7)(Biochemistry & Molecular Biology)
- Designing Potent Derivatives of Ovokinin(2-7), an Anti-hypertensive Peptide Derived from Ovalbumin
- Selective Proliferation of Lactic Acid Bacteria and Accumulation of Lactic Acid during Open Fermentation of Kitchen Refuse with Intermittent pH Adjustment
- Observation of Nuclear Resonance of Cu in Pb_2Sr_2Y_Ca_xCu_3O_With x=O and 0.5
- Safety Assessment of Genetically Engineered Food : Detection and Monitoring of Glyphosate-Tolerant Soybeans
- Hypocholesterolemic Activity of β-Lactotensin As a Neurotensin Receptor Agonist
- Application of Shielded Hot-Wire Viscosity Sensor to Monitoring Cultivations of Xanthomonas campestris and Nicotiana tabacum BY-2
- Release of Hemorphin-5 from Human Hemoglobin by Pancreatic Elastase(Food & Nutrition Science)
- Characterization of β-Lactotensin, a Bioactive Peptide Derived from Bovine β-Lactoglobulin, as a Neurotensin Agonist(Food & Nutrition Science)
- Enterostatin (VPDPR) Has Anti-analgesic and Anti-amnesic Activities
- Interactions During Heat-induced Gelation in a Mixed System of Soybean 7S and 11S Globulins(Food & Nutrition)
- Mechanism of Heat-Induced Gelation and Gel Properties of Soybean 7S Globulin(Food & Nutrition)
- Crystallization and Preliminary X-Ray Analysis of Soybean Proglycinins Modified by Protein Engineering
- Safety Assessment of Transgenic Potatoes with Soybean Glycinin by Feeding Studies in Rats
- Soybean β-Conglycinin Diet Suppresses Serum Triglyceride Levels in Normal and Genetically Obese Mice by Induction of β-Oxidation, Downregulation of Fatty Acid Synthase, and Inhibition of Triglyceride Absorption
- Introduction of DPR, an Enterostatin Fragment Peptide, into Soybean β-Conglycinin α' Subunit by Site-directed Mutagenesis
- Functions of Characteristic Cys-Gly-His-Cys (CGHC) and Gln-Glu-Asp-Leu (QEDL) Motifs of Microsomal ER-60 Protease
- Gelling Properties of Soybean β-Conglycinin Having Different Subunit Compositions
- Design of Highly Functional Soybean Protein by Genetic Introduction of Anti-hypertensive Peptide
- Ovokinin is an Agonist for Angiotensin AT_2 Receptors
- Potentiation of the Antihypertensive Activity of Orally Administered Ovokinin, a Vasorelaxing Peptide Derived from Ovalbumin, by Emulsification in Egg Phosphatidyl-choline
- Human Enterostatin (APGPR) Stimulates Learning Performance
- Effect of Amplification of Desensitized purF and prs on Inosine Accumulation in Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Effects of xapA and guaA Disruption on Inosine Accumulation in Escherichia coli
- Effects of edd and pgi Disruptions on Inosine Accumulation in Escherichia coli
- Enterostatin (VPDPR) and Its Peptide Fragment DPR Reduce Serum Cholesterol Levels after Oral Administration in Mice(Food & Nutrition Science)
- gsk Disruption Leads to Guanosine Accumulation in Escherichia coli
- Introduction of a Low Molecular Weight Agonist Peptide for Complement C3a Receptor into Soybean Proglycinin A_B_ Subunit by Site-directed Mutagenesis
- Adenine Deaminase Activity of the yicP Gene Product of Escherichia coli
- Introduction of Enterostatin (VPDPR) and a Related Sequence into Soybean Proglycinin A_B_ Subunit by Site-directed Mutagenesis
- Royal Jelly from Apis cerana japonica and Apis mellifera
- Effect of Acetic Acid or Rice Vinegar Feeding after Exercise on Blood Glucose in Fasted and Running-trained Rats
- Shielded Hot-wire Viscosity Sensor On-line for a Flowing System Using a Shield of High Thermal Conductivity
- Production of D-Amino Acid Oxidase from Aspergillus sojae
- Cloning, Expression, and Nucleotide Sequence of the N-Acyl-_D-Aspartate Amidohydrolase Gene from Alcaligenes xylosoxydans subsp. sylosoxydans A-6
- β-Lactotensin, a Neurotensin NT_2 Agonist, Stimulates Bile Acid Secretion
- Physiological effects and mechanism of Arg-Ile-Tyr, a multifunctional peptide derived from rapeseed protein
- Antidiabetic Activity of Novokinin (RPLKPW) after Oral Administration in KK-A^y Mice
- Mechanism for Suppression of Food Intake by AT_2 Rreceptor Agonists
- Anti-Alopecia Effect of Gly-Leu-Phe, an Immunostimulating Peptide Derived from α-Lactalbumin
- Isolation and Characterization of Hypotensive Peptides from Enzymatic Digest of Repeseed Protein
- RPLKPW, a Designed Hypotensive Peptide Relaxes Artery and Stimulates Hair Growth via Angiotensin AT_2 Receptor
- High-level Expression, Purification and Functional Properties of Soybean Proglycinin from Escherichia coli(Biological Chemistry)
- Polymorphism and Expression of cDNAs Encoding Glycinin Subunits(Food & Nutrition)
- Network Structure and Forces Involved in Perilla Globulin Gelation: Comparison with Sesame Globulin
- Mechanism of the Protective Effect of Intraperitoneally Administered Agonists for Formyl Peptide Receptors against Chemotherapy-Induced Alopecia
- Mechanism of the Protective Effect of Intraperitoneally Administered Agonists for Formyl Peptide Receptors against Chemotherapy-Induced Alopecia
- Accumulation of the Bioactive Peptides, Novokinin, LPYPR and Rubiscolin, in Seeds of Genetically Modified Soybean
- A Potent Hypotensive Peptide, Novokinin, Induces Relaxation by AT_2- and IP-Receptor-Dependent Mechanism in the Mesenteric Artery from SHRs
- Subunit Structure and Functional Properties of the Predominant Globulin of Perilla (Perilla frutescens var. frutescens) Seeds
- Soymorphins, Novel μ Opioid Peptides Derived from Soy β-Conglycinin β-Subunit, Have Anxiolytic Activities
- Involvment of σ_1 and Dopamine D_1 Receptors in Anxiolytic Effect of Rubiscolin-6, a δ Opioid Peptide Derived from Spinach Rubisco
- Isolation and Characterization of a New Opioid Peptide Soymorphin Derived from Soy β-Conglycinin
- β-Lactotensin (His-Ile-Arg-Leu) Derived from Bovine β-Lactoglobulin Suppresses Food Intake after Oral Administration in Mice
- Modification Tolerability of Soybean Proglycinin
- Soymorphin-5, a soy-derived μ opioid peptide, improves glucose and lipid metabolism in diabetic KK-Ay mice
- Ileum-contracting Peptides Derived from Human Lactoferrin
- Anti-alopecia Mechanism of Soymetide-4
- Design of Soymetide-4 Derivatives to Potentiate the Anti-alopecia Effect
- Network Structure and Forces Involved in Perilla Globulin Gelation : Comparison with Sesame Globulin
- A Hair Growth-Promoting Effect of Chinese Black Tea Extract in Mice