Cloning and Sequence Analysis of cDNA encoding Rhizopus niveus Lipase
スポンサーリンク
概要
- 論文の詳細を見る
Complementary DNA encoding Rhizopus niveus lipase (RNL) was isolated from the R. niveus IF04759 cDNA library using a synthetic oligonucleotide corresponding to the amino acid sequence of the enzyme. A clone, which had an insert of 1.0 kilobase pairs, was found to contain the coding region of the enzyme. The lipase gene was expressed in Escherichia coli as a lacZ fusion protein. The mature RNL consisted of 297 amino acid residues with a molecular mass of 32 kDa. The RNL sequence showed significant overall homology to Rhizomucor miehei lipase and the putative active site residues were strictly conserved.
- 社団法人日本農芸化学会の論文
- 1992-05-23
著者
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KOHNO Mitsutaka
Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd.
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KUGIMIYA Wataru
Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd.
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Hashimoto Yukio
Central Research Institute Tsukuba R & D Center, Fuji Oil Co., Ltd.
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Hashimoto Y
Novelty Materials Research Institute Hannan R&d Center Fuji Oil Co.ltd.
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OTANI Yasuo
Central Research Institute, Fuji Oil Co., Ltd.
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Otani Yasuo
Central Research Institute Fuji Oil Co. Ltd.
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Kohno M
Food Science Research Institute Fuji Oil Co. Ltd.
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Kohno Mitsutaka
Central Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.
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Kugimiya W
New Ingredients Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.
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Kugimiya Wataru
Central Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.
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