Purification and Characterization of Intracellular Proteinases in Pleurotus ostreatus Fruiting Bodies
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概要
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A serine proteinase (ProA, EC 3.4.22.9) and two metalloendopeptidases (ProB, EC 3.4.99.32 and ProC, 3.4.24.4), have been puritied to homogeneity from the fruiting bodies of Pleurotus ostreatus. ProA is a serine proteinase with a mass of 30kDa, which has amidolytic and esterolytic activities besides proteolysis and catalyzes preferential cleavage of the peptide bonds involving the carboxyl groups of hydrophobic amino acid residues in oxidized bovine insulin B chain. The N-terminal amino acid sequence was VTQTNAPWGLSRL. ProB is a zinc-enzyme with a mass of 18kDa, which is devoid of lysine, and its N-terminal sequence was ATFVGCSATRQ. The enzyme is inactivated completely by EDTA and 1,10-phenanthroline, and Zn^<2+>-depleted ProB can regain the activity with Zn^<2+>, Co^<2+>, or Mn^<2+>. Specitic cleavage of Pro_<29>-Lys_<30> in oxidized bovine insulin B chain, preferential generation of lysylpeptides from proteins, and a high susceptibility of polylysine suggest that ProB splits specifically the peptide bonds involving the α-amino group of lysyl residues. ProC is a metalloendopeptidase of a mass of 42.5KDa, and Zn^<2+> was the most effective divalent metal ion to activate the EDTA-inactivated enzyme.
- 社団法人日本農芸化学会の論文
- 1995-11-23
著者
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Dohmae N
Riken Saitama Jpn
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Dohmae Naoshi
Biomolecular Characterization Team Advanced Technol. Support Div. Riken Advanced Sci. Inst.
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Dohmae N
Riken(inst. Physical And Chemical Res.) Saitama Jpn
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TSUMURAYA Yoichi
Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University
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Hayashi Kenichi
Department of Applied Science, Kyushu University
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Dohmae Naoshi
Department of Biochemistry, Faculty of Science, Saitama University
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Miki Kiyoshi
Department of Biochemistry, Faculty of Science, Saitama University
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Hashimoto Yohichi
Department of Biochemistry, Faculty of Science, Saitama University
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Tsumuraya Yoichi
Department Of Bilchemistry Faculty Of Science Saitama University
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Miki Kiyoshi
Department Of Biochemistry Faculty Of Science Saitama University
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Tsumuraya Y
Saitama Univ. Saitama Jpn
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Hashimoto Y
Novelty Materials Research Institute Hannan R&d Center Fuji Oil Co.ltd.
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Hayashi Kenichi
Department Of Applied Physics Faculty Of Engineering Kyushu University
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Hayashi Kenichi
Department Of Biochemistry Faculty Of Science Saitama University
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