Synthesis of Maltosyl(α1→6)cyclodextrins through the Reverse Reaction of Thermostable Bacillus acidopullulyticus Pullulanase(Biological Chemistry)

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概要

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• Maltosyl(α1→6)α-, β- or γ-cyclodextrin was synthesized from maltose and α-, β- or γ-cyclodextrin, respectively, using Bacillus atidopullulyticus pullulanase (EC 3.2.1.41). More than 40% of each cyclodextrin substrate was converted to the corresponding maltosyl(α1→6)cyclodextrin under the conditions given below ; the combined concentration of maltose and cyclodextrin was 70〜75% (w/w), the molar ratio of maltose to cyclodextrin was 9〜18, and the amount of pullulanase was 100〜200units/g of cyclodextrin. The optimum pH and temperature for the formation of maltosyl(α1→6)cyclodextrins were 4.0〜4.5 and 60〜70℃, respectively. Each maltosyl(α1→6)-cyclodextrin produced was separated from noncyclic saccharides, maltose and branched tetraose, by methanol and ethanol precipitations. The maltosyl(α1→6)cyclodextrins were further purified by gel filtration on a Toyopearl HW 40S column and crystallization from aqueous (for maltosyl(α1→6)α-cyclodextrin) or methanol (for maltosyl(α1→6)β-cyclodextrin) solution. From 10 g each of the corresponding cyclodextrin, the yields of the purified maltosyl(α1→6)α-, β- and γ-cylcodextrins were 3.0〜3.6g, 2.5〜2.8g and 2.2〜2.5g, respectively. Identification of the maltosyl(α1→6)cyclodextrins was performed by means of hydrolysis with Klebsiella pneumonias pullulanase, methylation analysis and ^<13>C-NMR analysis.

• 社団法人日本農芸化学会の論文
• 1989-08-23

著者

• Tsumuraya Yoichi

  Department Of Biochemistry Faculty Of Science Saitama University

• Tsumuraya Yoichi

  Department Of Bilchemistry Faculty Of Science Saitama University

• SAKANO Yoshiyuki

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• Kusano Shuichi

  (present Address)ohmiya Research Laboratory Nikken Chemicals Co. Ltd.

• Kusano S

  Research Institute Fuji Sangyo Co. Ltd.

• Shiraishi T

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• SHIRAISHI Takanori

  Laboratory of Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Univ

• KUSANO Shuichi

  Laboratory of Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Univ

• Sakano Y

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Sakano Yoshiyuki

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Tokyo University Of Agriculture And

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