Purification and Properties of Bacillus acidopullulyticus Pullulanase (Biological Chemistry)

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概要

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• Two active forms (F1 and F2) of Bacillus acidopullulyticus pullulanase (EC 3.2.1.41 pullulan 6-glucanohydrolase) from promozyme 200L (Novo Industri A/S) were purified using 50% (v/v) acetone precipitation and chromatographies on CM-Toyopearl 650S, Toyopearl HW-55S, and Butyl-Toyopearl 650S columns. The F1 and F2 forms showed single bands in polycrylamide gel electrophoresis with or without sodium dodecyl sulfate. The optimum pHs and specific activities of F1 and F2 were about 5.0 and 200〜220 units/mg, respectively. The molecular weights of F1 and F2 were estimated as 115.000 and 116,000 and their isoelectric points were 5.0 and 5.2. The Km values of F1 and F2 for pullulan were the same, 2.5 mM glucose equivalents, and their k. values were calculated as 2,9×10^3 and 3.6×10^3s^<-1>, respectively, from the Line weaver-Burk plots. These K. values of F1 and F2 for pullulan are larger than that (2.9 × 10^2 sec^<-1>) of Klebsiella pneumoniae pullulanase. The activities of F1 and F2 on glycogen β-limit dextrins were 26〜46% of those of pullulan, but the activity of K. pneumoniae pullulanase on glycogen β-limit dextrins was 3〜6% of that on pullulan.

• 社団法人日本農芸化学会の論文
• 1988-09-23

著者

• TAKAHASHI Shin-Ichiro

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• SAKANO Yoshiyuki

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• FUJIMOTO Daisaburo

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• Kusano Shuichi

  (present Address)ohmiya Research Laboratory Nikken Chemicals Co. Ltd.

• Kusano S

  Research Institute Fuji Sangyo Co. Ltd.

• Shiraishi T

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• KUSANO Shuichi

  Laboratory of Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Univ

• NAGAHATA Naokazu

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• Sakano Y

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Sakano Yoshiyuki

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Tokyo University Of Agriculture And

• Nagahata Naokazu

  Laboratory Of Biological Chemistry Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo

• Fujimoto D

  Tokyo Univ. Agriculture And Technol. Tokyo Jpn

• Fujimoto Daisaburo

  Laboratory Of Biological Chemistry Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And Technology

• TAKAHASHI Shin-Ichiro

  Laboratory of Animal Cell Regulation, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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