Preparation and Enzymatic Hydrolysis of Maltosyl-α-cyclodextrin
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概要
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Maltosyl-α-cyclodextrin (6-α-maltosylcyclomaltohexaose, M-CD) was prepared from maltose and α-cyclodextrin by the reverse action of Bacillus pullulanase, and the action of α-amylases on this dextrin was examined. Among α-amylases tested, Thermoactinomyces vulgaris α-amylase (TVA) and Taka-amylase A (TAA) were found to attack the M-CD. Their action pattern on M-CD was studied. These α-amylases cleaved, first the cyclodextrin ring of M-CD, and the branched octasaccharides formed were immediately degraded to form glucose, branched tetraose, or pentaose, though the action pattern was different for TVA and TAA. In addition, TAA also split M-CD into glucose and glucosyl-α-cyclodextrin. Fission products at various stages of the reaction were separated and analyzed by paper chromatography and high performance liquid chromatography, their structures were analyzed, and the degradation pattern of M-CD was found.
著者
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KOBAYASHI Tsuneo
Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To
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Sakano Yoshiyuki
Laboratory Of Biochemistry Department Of Agricultural Chemistry Tokyo University Of Agriculture And
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SANO Mutsumi
Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Noko University
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KOBAYASHI Tsuneo
Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo Noko University
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KOBAYASHI Tsuneo
Laboratory of Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology
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