Immobilization of Bacillus acidopullulyticus Pullulanase and Properties of the Immobilized Pullulanases

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概要

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• In order to make an enzyme reactor for the production of maltosyl (α1→6) cyclodextrins (G_2-CD_s), Bacillus acidopullulyticus pullulanase (EC 3.2.1.41,Pase) was immobilized on various carriers. Different methods of immobilization were employed, including physical adsorption, cross-linking and ionic binding, and the enzymic properties of immobilized Pases were investigated. The immobilized enzymes prepared by adsorption on porous glass (PG-Pase), covalent binding on chitosan beads treated with glutaraldehyde (GA-CB-Pase) and ionic binding on Amberlite IRC-50 (IRC-Pase) had high Pase activities. The optimum pH of Pase shifted from 5.0,which is optimum for the native B. acidopullulyticus enzyme, to 3.5 for IRC-Pase and GA-CB-Pase, and 5.5 for PG-Pase. K_m values for pollulan- and G_2-α-CD-hydrolyzing activities of PG-, GA-CB- and IRC-Pases were greater than those of native Pase. The ratio of G_2-α-CD-hydrolyzing activity to pullulan-hydrolyzing activity in immobilized Pase was higher than that of native Pase. The G_2-α-CD-synthesizing activities of column reactors (1.6×10cm) packed with PG-and GA-CB-Pases hardly decreased after continous operation for 72d at 60℃, but the G_2-α-CD-synthesizing activity of the IRC-Pase column reactor devreased to about 50% of the initial activity after 30 d-operation (60℃).

• 公益社団法人日本生物工学会の論文
• 1989-10-25

著者

• TAKAHASHI Shin-Ichiro

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• SAKANO Yoshiyuki

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• FUJIMOTO Daisaburo

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• Kusano Shuichi

  (present Address)ohmiya Research Laboratory Nikken Chemicals Co. Ltd.

• Kusano S

  Research Institute Fuji Sangyo Co. Ltd.

• Shiraishi T

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• SHIRAISHI TAKANORI

  (Present address)Ohmiya Research Laboratory, Nikken Chemicals Co. Ltd.,

• Sakano Y

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Sakano Yoshiyuki

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Tokyo University Of Agriculture And

• Fujimoto D

  Tokyo Univ. Agriculture And Technol. Tokyo Jpn

• Fujimoto Daisaburo

  Laboratory Of Biological Chemistry Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And Technology

• TAKAHASHI Shin-Ichiro

  Laboratory of Animal Cell Regulation, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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