A 110-kDa WGA-binding Glycoprotein Involved in Cell Adhesion Acts as a Receptor for Aggregation Factor in Embryos of the Sea Urchin, Hemicentrotus pulcherrimus(Developmental Biology)
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概要
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Glycoproteins that bind wheat germ agglutinin (WGA-binding glycoproteins) were isolated from a Triton extract of an acetone powder of blastula embryos of the sea urchin, Hemicentrotus pulcherrimus. The Triton extract was brought to 30% saturation with ammonium sulfate. The supernatant showed inhibitory activity directed against hemagglutination caused by WGA. The supernatant was applied to a column of WGA-agarose and the bound fraction was eluted with N-acetyl-D-glucosamine. The fraction that bound to WGA-agarose was applied to a column of Sephacryl S-400 after reduction and alkylation. After fractionation on Sephacryl S-400, four WGA-binding glycoproteins were identified with molecular weights of 110-, 70-, 66- and 60-kDa after ectrophoresis on sodium dodecyl sulfate polyacrylamide gels and staining with WGA labeled with horseradish peroxidase (HRPO). Staining with HRPO-labeled aggregation factor (AF) showed that, of these proteins, only the 110-kDa glycoprotein bound to AF. Moreover, the trypsin fragments of the 110-kDa glycoprotein inhibited the aggregation of dissociated cells of sea urchin embryos caused by AF. The sugar composition of the 110-kDa glycoprotein indicated that this protein contained high levels of a mannose-type oligosaccharide. These results suggest that the 110-kDa WGA-binding glycoprotein on the cell surface may be involved in cell adhesion as a receptor for AF, to which it binds by sugar-lectin type interactions.
- 社団法人日本動物学会の論文
- 1991-02-15
著者
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Suyemitsu Takashi
Department of Regulation Biology, Faculty of Science, Saitama University
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TSUMURAYA Yoichi
Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University
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Tsumuraya Yoichi
Department Of Bilchemistry Faculty Of Science Saitama University
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Tsumuraya Yoichi
Department Of Biochemistry Saitama University
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KAMIMURA JUN
Department of Regulation Biology, Saitama University
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TONEGAWA YASUTO
Department of Regulation Biology, Saitama University
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Kamimura Jun
Department Of Regulation Biology Saitama University:(present Address)department Of Rheumatology Inst
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Suyemitsu Takashi
Department Of Regulation Biology Faculty Of Science Saitama University
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Suyemitsu Takashi
Department Of Regulation Biology Saitama University
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Tonegawa Yasuto
Department Of Regulation Biology Saitama University
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Tonegawa Yasuto
Department Of Regulation Biology Faculty Of Science Saitama University
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