Effects of Exogastrula-Inducing Peptides on Cell Proliferation in Embryos of the Sea Urchin Anthocidaris crassispina
スポンサーリンク
概要
- 論文の詳細を見る
The effects were examined of exogastrula-inducing peptides (EGIPs), found in embryos of the sea urchin Anthocidaris crassispina and known to be homologous to EGF, on cell proliferation in restricted areas of embryos at the early stages of development. Blastomeres of embryos were mechanically separated into macromeres and mesomeres at the 16-cell stage. Although the number of isolated blastomeres increased linearly in the culture system in vitro, EGIPs inhibited or retarded the division of macromeres during the period between 16 hr and 20 hr after fertilization, which corresponded to the times of gastrulation in normal embryos, but they did not affect the division of mesomeres. These differences in the proliferation of blastomeres were confirmed immunohistochemically with a BrdU-specific antibody on whole embryos. Furthermore, the distribution of the cytoskeleton in embryos during early development was examined with a tubulin-specific antibody for microtubules and with NBD-phallacidin for microfilaments. We found local differences in the distribution of cytoskeletal elements of a normal embryo, as well as differences between normal and EGIPs-treated embryos. These results suggest that EGIPs inhibit or retard the division of the progeny of macromeres, namely, the blastomeres of the vegetal-plate area, via a disturbance in the development of the cytoskeleton at the early gastrula stage, leading to exogastrulation.
- 社団法人日本動物学会の論文
- 1993-10-15
著者
-
FUJITA Yoshiaki
Department of Nutrition, Tokyo Metropolitan Institute of Gerontology
-
Fujita Y
Department Of Regulation Biology Saitama University
-
Fujita Yoshiaki
Department Of Clinical Nutrition Faculty Of Health Science And Technology Kawasaki University Of Med
-
Yamasu Kyo
Department of Regulation Biology, Faculty of Science, Saitama University
-
Suyemitsu Takashi
Department of Regulation Biology, Faculty of Science, Saitama University
-
FUJISAWA Hirosuke
Department of Neurosurgery, School of Medicine, Yamaguchi University
-
Ishihara Katsutoshi
Department of Regulation Biology, Saitama University
-
Igarashi Shin-ichi
Department of Regulation Biology, Faculty of Science, Saitama University
-
Yamasu K
Department Of Regulation Biology Faculty Of Science Saitama University
-
Yamasu Kyo
Department Of Life Science Graduate School Of Science And Engineering Saitama University
-
Suyemitsu T
Saitama Univ. Saitama Jpn
-
Suyemitsu Takashi
Department Of Regulation Biology Graduate School Of Science And Engineering Master Couse Saitama Uni
-
Fujisawa Hirosuke
Department Of Biology Faculty Of Education Saitama University
-
Suyemitsu Takashi
Department Of Regulation Biology Faculty Of Science Saitama University
-
Igarashi Shin-ichi
Department Of Regulation Biology Faculty Of Science Saitama University
-
Ishihara Katsutoshi
Department Of Biochemistry Saitama University
-
Ishihara Katsutoshi
Department of Regulation Biology, Faculty of Science, Saitama University
-
Igarashi Shin-ichi
Department of Applied Chemistry, Faculty of Engineering, Yamagata University
関連論文
- Disappearance of Diurnal Rhythm of Energy Expenditure in Genetically Diabetic Obese Rats
- Physical Activities and Energy Expenditures of Institutionalized Japanese Elderly Women
- Measurement of Carbon Monoxide in Exhaled Breath as a Possible Marker of Stress
- LOCALIZATION OF SYNDECAN IN EMBRYOS OF THE SEA URCHIN ANTHOCIDARIS CRASSISPINA(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Role of syndecan in the elongation of postoral arms in sea urchin larvae
- ROLE OF SYNDECAN IN THE ELONGATION OF POSTORAL ARMS IN PLUTEUS LARVAE(Developmental Biology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Cloning and characterization of cDNA for syndecan core protein in sea urchin embryos
- Association of the sea urchin EGF-related peptide, EGIP-D, with fasciclin I-related ECM proteins from the sea urchin Anthocidaris crassispina
- Anomalous Origin of the Anterior Cerebral Artery and Congenital Skull Dysplasia : Case Report
- Purification of EGIP-D-Binding Protein from the Embryos of the Sea Urchin Anthocidaris crassispina
- Local Change of an Exogastrula-Inducing Peptide (EGIP) in the Pluteus Larva of the Sea Urchin Anthocidaris crassispina
- Effects of Exogastrula-Inducing Peptides on Cell Proliferation in Embryos of the Sea Urchin Anthocidaris crassispina
- Various Magnetic Resonance Imaging Patterns of Chronic Subdural Hematomas : Indicators of the Pathogenesis?
- Changes in Nitric Oxide Synthesis and Epileptic Activity in the Contralateral Hippocampus of Rats Following Intrahippocampal Kainate Injection
- Expression of a src-type protein tyrosine kinase gene, AcSrc1, in the sea urchin embryo
- The Protein Tyrosine Kinases of the Sea Urchin Anthocidaris Crassispina
- Sliding thin slab, minimum intensity projection of the lung in asymptomatic subjects : lower limits of lung attenuation, without airways
- HRCT-Pathological Correlation in a Case of UIP Deterioration
- Induction of metamorphosis in the sand dollar Peronella japonica by thyroid hormones
- A 110-kDa WGA-binding Glycoprotein Involved in Cell Adhesion Acts as a Receptor for Aggregation Factor in Embryos of the Sea Urchin, Hemicentrotus pulcherrimus(Developmental Biology)
- Genomic organization, alternative splicing, and multiple regulatory regions of the zebrafish fgf8 gene
- Functional organization of DNA elements regulating SM30α, a spicule matrix gene of sea urchin embryos
- N-Acetyl-D-Galactosamine-Specific Hemagglutinin Purified from Seminal Plasma of the Sea Urchin, Hemicentrotus pulcherrimus(Biochemistry)
- Protein malnutrition in elderly patients with chronic obstructive pulmonary disease
- Similarities between the Primary Structures of Exogastrula-Inducing Peptides and Peptide B Purified from Embryos of the Sea Urchin,Anthocidaris crassispina
- Neuroprotective Effects of Ebselen Following Forebrain Ischemia : Involvement of Glutamate and Nitric Oxide
- OBITUARY--Juro Ishida(1908-1994)〔含 肖像〕
- Chemical Composition of the Egg Membrane of the Toad
- Activation of Glycolytic Process at the Time of Fertilization in Sea Urchin Eggs
- Significance of Differences Between Brain Temperature and Core Temperature (Delta T) During Mild Hypothermia in Patients With Diffuse Axonal Injury
- Expression of the Gene for Translation Elongation Factor 1α-Related Protein during Development of the Sea Urchin Anthocidaris crassispina(Developmental Biology)
- Binding Properties of Thyroxine to Nuclear Extract from Sea Urchin Larvae
- CLONING OF HEMICENTROTUS PULCHERRIMUS DOPAMINE RECEPTOR D1(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Progression of Stenosis Into Occlusion of the Distal Posterior Cerebral Artery Supplying an Occipital Arteriovenous Malformation Manifesting as Multiple Ischemic Attacks:—Case Report—
- p.Arg332Cys Mutation of NOTCH3 Gene in Two Unrelated Japanese Families with CADASIL
- External Carotid Artery Stenting and Superficial Temporal Artery to Middle Cerebral Artery Anastomosis for Internal Carotid Artery Occlusion With External Carotid Artery Severe Stenosis -Case Report-
- Progression of Stenosis Into Occlusion of the Distal Posterior Cerebral Artery Supplying an Occipital Arteriovenous Malformation Manifesting as Multiple Ischemic Attacks -Case Report-
- Reactions of 2-chlorothiophene with cation exchange resin and 100% orthophosphoric acid. Formation of the dimer type products containing a tetrahydro-2-thiophenone moiety.