Expression of the Gene for Translation Elongation Factor 1α-Related Protein during Development of the Sea Urchin Anthocidaris crassispina(Developmental Biology)

概要

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The cDNA for a protein that is related to translation elongation factor 1α (EF-1α) has been cloned from the sea urchin Anthocidaris crassispina. The sea urchin EF-1α-related protein (AcEFP) seems somewhat unique in structure compared to EF-1α of other organisms so that it is uncertain if AcEFP is a genuine EF-1α. Still, it possesses many features of typical EF-1α reported so far, suggesting that AcEFP is involved in protein synthesis or its regulation. Genomic Southern analysis indicated that the sea urchin genome contains one or at most two copies of the AcEFP gene. A single transcript of 2.2 kb is expressed ubiquitously in adult tissues examined and, during embryogenesis, zygotically after the blastula stage. Whole mount in situ hybridization showed that the AcEFP gene is also widely expressed in embryos, with relatively high expression in the gut and oral ectoderm, both of which are proliferating tissues in embryos. The expression of AcEFP was not affected in embryos by agents that destabilize the extracellular matrix (ECM), suggesting that expression of AcEFP is relatively independent of the integrity of ECM in sea urchin embryos.
社団法人日本動物学会の論文
1999-10-15