Cloning of Genomic DNA of Rhizopus niveus Lipase and Expression in the Yeast Saccharomyces cerevisiae

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概要

• 論文の詳細を見る

• Genomic DNA encoding Lipase I was cloned from Rhizopus niveus strain IFO4759. For expression of this gene in S. cerevisiae, DBY746 was transformed with YEp352PLipS, which had the cloned lipase gene under the control of a PGK promoter. This strain secreted the lipase at a high level (350 U/ml). The strain ND-12B was produced by a mating of DBY746,harboring YEp352PLipS, and NA74-3A, and dissection of asci. This new strain secreted the lipase up to 530 U/ml. Moreover, the lipase was produced most effectively in a medium containing Bacto-yeast extract, soypeptide, and sucrose.

• 社団法人日本農芸化学会の論文
• 1998-12-23

著者

• KOHNO Mitsutaka

  Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd.

• KUGIMIYA Wataru

  Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd.

• Kohno Mitsutaka

  Central Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.

• ENATSU Makoto

  Central Research Institute, Tsukuba R&D Center, Fuji Oil Co., Ltd.

• Kugimiya Wataru

  Central Research Institute Tsukuba R&d Center Fuji Oil Co. Ltd.

• Enatsu M

  Fuji Oil Co. Ltd. Ibaraki Jpn

• Enatsu Makoto

  Central Research Institute, Tsukuba R&D Center, Fuji Oil Co., Ltd.

関連論文

• The Crystal Structure of Lipase II from Rhizopus niveus at 2.2 Å Resolution
• Purification, Characterization, and Crystallization of Two Types of Lipase from Rhizopus niveus
• Molecular Cloning and Structure of the Gene for Esterase from a Thermophilic Bacterium, Bacillus stearothermophilus IFO 12550
• Cloning and Sequence Analysis of cDNA encoding Rhizopus niveus Lipase
• Cloning of Genomic DNA of Rhizopus niveus Lipase and Expression in the Yeast Saccharomyces cerevisiae

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