Preparation of Biologically Active and Site Specifically Radioiodinated Recombinant Human Insulin-like Growth Factor-I
スポンサーリンク
概要
- 論文の詳細を見る
Recombinant human insulin-like growth factor-I (rhIGF-I) was iodinated using a lactoperoxidase-catalyzed labeling method. The labeled products were separated into more than five fractions by ion-paired reverse-phase high performance liquid chromatography (HPLC). A fraction (peak 1), which showed the highest yield and radiactivity, was found to be biologically active in the BALB/c 3Ts cell proliferating system. The site of the iodination was investigated by S-pyridylethylation followed by trypsinization and separation with HPLC using reverse phase columns. From the amino acid analysis of the peaks which were radioactive, the iodination site of peak 1 was revealed to be Try-24 and Try-60. This is the first report of the biological activity of radioactive peptide hormone with a defined laveled site.
- 社団法人日本薬学会の論文
- 1992-03-25
著者
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伊藤 正明
Faculty Of Pharmaceutical Sciences Osaka University
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栢森 裕三
国立循環器病センター
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小濱 靖弘
大阪大学 薬 微薬品化
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辻川 和丈
阪大薬
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小濱 靖弘
Faculty of Pharmaceutical Sciences, Osaka University
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辻川 和丈
Faculty of Pharmaceutical Sciences, Osaka University
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三村 務
Faculty of Pharmaceutical Sciences, Osaka University
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寺本 哲之
Faculty of Pharmaceutical Sciences, Osaka University
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岡部 勝
Faculty of Pharmaceutical Sciences, Osaka University
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OKA Hiroaki
Faculty of Pharmaceutical Sciences, Osaka University
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栢森 裕三
Faculty of Pharmaceutical Sciences, Osaka University
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三村 務
大阪大学薬学部
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岡部 勝
大阪大薬学部
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Tanaka K
Department Of Molecular Engineering Graduate School Of Engineering Kyoto University
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小浜 靖弘
大阪大学薬学部
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Oka H
Faculty Of Pharmaceutical Sciences Osaka University
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Oka Hiroaki
Faculty Of Pharmaceutical Sciences Osaka University
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Tanaka Ken-ichiro
Faculty Of Pharmaceutical Sciences Okayama University
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岡部 勝
大阪大学 薬
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寺本 哲之
Faculty Of Pharmaceutical Sciences Osaka University
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辻川 和丈
Faculty of Pharmaceutical Sciences, Okayama University
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