Streptomyces sp.の菌体から抗炎症成分としての脂肪酸類の分離と同定

概要

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Erythrocyte membrane stabilizing fraction (EMSF) was purified from the acetone extract of the mycelium of Streptomyces sp. F4818 by the combination of chromatography on silica gel and preparative thin-layer chromatography on silica gel. 1) Heat-induced hemolysis was significantly inhibited by EMSF at a concentration of 2 μg/ml and the hypotonic hemolysis was also inhibited by about 50% at a concentration of 12 μg/ml of EMSF. 2) EMSF showed high anti-inflammatory activity for carrageenin-induced edema in the hind paw of rats at the oral dose of 50 mg/kg. 3) It was proved that EMSF was a fatty acid mixture, consisting of C_<14 : 0>, iso- or anteiso-C_<14 : 0>, C_<15 : 0>, iso C_<15 : 0>, C_<16 : 0>, iso-C_<16 : 0>, iso- or anteiso-C_<17 : 0>, C_<18 : 0>, and C_<18 : 1> from the result of gas chromatography-mass spectra (GC-MS) analysis on their methyl esters. 4) Palmitic acid, the main fatty acid component in EMSF, markedly suppressed the heat-induced, hypotonic hemolysis in vitro and carrageenin-induced edema in vivo.
公益社団法人日本薬学会の論文
1977-11-25