ワクシニアウイルスで感染した家兎皮膚組織中の生物活性物質の研究特に胃酸分泌抑制物質の分離とその性質
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概要
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Methods are described for the isolation of biologically active substances from rabbit skin tissues infected with vaccinia virus. The inhibitory activity on tetragastrin-stimulated gastric acid secretion found in the alkaline aqueous extract of the infected tissues boiled in acetone was concentrated by precipitation with ammonium sulfate and acetone. Further purification of the active material was carried out by chromatography over DEAE-cellulose and gel filtration over Sephadex G-75. The yield of the purified protein, Fr. I, was 2.25 mg from 1.0 kg of infected tissues, and Fr. I showed 77.2±8.4% (n=5)of inhibitory activity when doses of 5.0 mg/kg were administered intravenously into rats. This protein was also a potent inhibitor of gastric acid, gastric juice, and pepsin secretion, and ulcers induced by pylorus ligation. Molecular weight of Fr. I was 69000 by sodium dodecyl sulfate gel electrophoresis and gel filtration through Sephadex G-75. Amino acid analysis demonstrated 18 different amino acids, with high content of glutamic acid and proline. Another biologically active material was extracted with boiled aqueous phenol and fractionated with Dowex 50W chromatography. This material, contained uracil, xanthine, several amino acid-related substances, and nucleoside-related substance. Fr. d. Ornithine, ethanolamine, and Fr. d inhibited heat-induced hemolysis of rabbit erythrocytes, and Fr. d was also a potent inhibitor of chemotaxis of rabbit polymorphonuclei in vitro.
- 公益社団法人日本薬学会の論文
- 1976-10-25
著者
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青沼 繁
大阪大学薬学部
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小浜 靖弘
大阪大学薬学部
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陳 英俊
大阪大学薬学部
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屋敷 伸治
大阪大学薬学部
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青沼 繁
Faculty Of Pharmaceutical Sciences Osaka University
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大西 昇
Faculty of Pharmaceutical Sciences, Osaka University
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江川 宏
大阪大学薬学部
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大西 昇
大阪大学薬学部
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大西 昇
Faculty Of Pharmaceutical Sciences Osaka University
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