Streptomyces bottropensis F4708の培養濾液から胃液分泌抑制物質の精製とその性質

概要

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In the course of screening studies on new substances inhibiting gastric juice secretion among metabolites of Streptomyces, Streptomyces bottropensis F4708 was found to produce a new principle in its culture filtrate inhibiting gastric juice secretion. A crude active fraction was obtained as an isoelectric precipitate by adjusting the culture filtrate to pH 3.0 and then defatted. The lipid-free active fraction was purified by chromatography on hydroxylapatite column and gel filtration on Sephadex G-100 column. The purified preparation was named gastric juice inhibitory substance. From the result if chemical analysis, this substance was considered to be a kind of glycoprotein. At the dose of 1.0 mg/kg (i-p.), this substance markedly decreased gastric juice volume, total acid output, and total peptic activity in pylorus-ligated rats. Furthermore, it showed an inhibitory effect on the gastric acid secretion stimulated by tetragastrin or histamine in the perfused stomach preparation of rats, and also inhibited ulceration developed in the forestomach of pylorus-ligated rats. However, antagonism between this substance and acetylcholine was not recognized on the contraction of guinea-pig ileum.
公益社団法人日本薬学会の論文
1976-05-25