^<131>I-標識Monoiodohistidineを利用したカルノシンおよび関連N-α-Aminoacylhistidineの生合成 : 心臓の研究第11報

概要

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5-Monoiodohistidine was synthesized and its conjugation with β-alanine. L-aspartic acid, L-glutamic acid, L-leucine, and DL-β-aminoisobutyric acid was followed in vitro with radiolabeled 5-monoiodohistidine-^<131>I as the substrate. It was found that only β-alanine underwent conjugation, as in the case of using ^<14>C-labeled β-alanine and histidine, and biosynthesis of ^<131>I-carnosine was observed. This reaction is markedly proceeded by the enzyme system from the liver and kidney, and weakly by that from skeletalmuscle and small intestine, and this enzyme preparation is very labile in any case. This experiment seems to suggest that the assay of the ability for biosynthesis of carnosine by the use of ^<131>I-labeled monoiodohistidine would be facile and it may be considered that the final step in the biosynthesis of carnosine in vivo is the β-peptide bonding between β-alanine and histidine.
社団法人日本薬学会の論文
1968-01-25