Studies on Sperm Capacitation. V. Characterization of Decapacitation Factor from Guinea-pig Spermatozoa
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概要
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Fr. I from guinea-pig spermatozoa which had DF activity against rabbit spermatozoa showed inhibitory effect on respiration and motility of guinea-pig spermatozoa. Measurement of DF activity was attempted in mouse in vitro fertilization system and the potent inhibition of sperm penetration into ova by Fr. I was confirmed, while the addition of anti-Fr. I-immunoglobulin (anti-Fr. I-Ig.) caused disappearance of fertilization inhibitory activity of Fr. I. The DF activity of Fr. I was partially destroyed during incubation at 60° for 2 hours and completely destroyed at 80° for 2 hours. Alkaline and acid treatment of Fr. I caused no effect on the DF activity. The Fr. I did not decrease its DF activity by the digestion with β-amylase. From the enzymatic point of view the inhibition of corona radiata penetrating enzyme by Fr. I was noted.
- 公益社団法人日本薬学会の論文
- 1976-05-25
著者
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河合 裕一
Faculty of Pharmaceutical Sciences
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青沼 繁
Faculty of Pharmaceutical Sciences of Kinki University
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岡部 勝
Faculty of Pharmaceutical Sciences, Osaka University
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岡部 勝
大阪大薬学部
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河合 裕一
Faculty Of Pharmaceutical Sciences Osaka University
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松本 堅志
Research Laboratories, Dainippon Pharmaceutical Co., Ltd.,
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青沼 繁
Faculty Of Pharmaceutical Sciences Osaka University
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鈴木 宏治
Faculty of Pharmaceutical Sciences, Osaka University
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松本 堅志
Faculty of Pharmaceutical Sciences, Osaka University
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松本 堅志
Research Laboratories Dainippon Pharmaceutical Co. Ltd.
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鈴木 宏治
Faculty Of Pharmaceutical Sciences Osaka University
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岡部 勝
大阪大学 薬
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