Bacillus属細菌の産生する生理活性物質の研究(第5報)B.subtilis Kにより産生される血清カルシウム低下性物質α因子より界面活性成分と脂質の除去

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It is found out that the α-factor, which is a serum calcium-decreasing substance produced by Bacillus subtilis K, contains a cytolytic factor which causes cytolysis of protoplasts and mammalian red blood cells. This cytolytic factor was isolated from the α-factor through the procedures, such as precipitation by CaCl_2,extraction with ether, gel filtration over Sephadex LH-20,and so on. From comparison with a sample of Surfactin, the chemical principle of the isolated cytolytic factor was identified with Surfactin or its analog, which is a surface-active peptide lipid. The peptide lipid was liberated and removed from lipoprotein α-I through gel filtration of the α-factor in the presence of Mg^<2+> or Ca^<2+>. Serum calcium-decreasing activity was found out to be in α-I, and Surfactin or its analog had only a weak activity. Through gel filtration of lipoprotein α-I in the presence of sodium deoxycholate apolipoprotein (Apo α-I) with serum calcium decreasing activity was obtained. From these results, the protein moiety of α-I is considered to be necessary for the appearance of its biological activity. The molecular weight of apo-α-I was measured by polyacrylamide gel disc electrophoresis in the presence of sodium dodecyl sulfate and calculated to be about 65000.
公益社団法人日本薬学会の論文
1974-04-25