心臓の研究(第16報)胸腺中の生理活性物質-特に牛胸腺から心臓機能促進物質の分離とその性質

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During studies on the humoral factor affecting heart function, three different lipoproteins promoting the function of a frog heart, Fr. I-A, Fr. I-B, and Fr. II, were obtained from the calf thymus by extraction with 2 N acetic acid, followed by column chromatography over Sephadex G-25,Sephadex G-50,CM-cellulose, and DEAE-cellulose. The N-terminal amino acids in them were lysine, lysine and glutamic acid, and their molecular weights were 17500,17500,and 3000,respectively. These lipoproteins increased the ^<32>P-uptake into chick myocardium 2 hr after intraperitoneal injection of 0.5 or 2.0 mg/chick, but did not show any effect on rabbit blood pressure and the kallikrein-like arginine esterase activity. Active principles in molecule of these lipoproteins seemed to be a protein, not lipid. The biological and physico-chemical differences between heart-promoting substances from calf thymus and from myocardium were discussed. Another lipoprotein, Fr. III, promoting an antibody production competence was isolated from the calf thymus. Fr. III increased a hemolytic titer, plaque-forming cells of the spleen, and respiration of spleen cells in neonatally thymectomized mice. The rabbit antiserum against extract from mouse thymus, which possessed the same activity as Fr. III, inhibited the antibody production competence of mouse in vivo.
公益社団法人日本薬学会の論文
1976-09-25