Molecular Cloning and Characterization of a Cysteine-rich 16.6-kDa Prolamin in Rice Seeds

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概要

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• An alcohol-soluble storage protein, a 16.6-kDa prolamin found in rice seeds, was purified from both the total protein body and purified type I protein body fractions. The partial amino acid sequences of three tryptic peptides generated from the purified polypeptide were analyzed. A part of the 16.6-kDa prolamin cDNA was amplified from developing seed mRNA by the reverse transcribed polymerase chain reaction using an oligo (dT) primer and a primer which was synthesized based on the partial amino acid sequence. The amplified product was used to isolate the fulllength cDNA close (λRP16) from a developing seed cDNA library. The cDNA has an open reading frame encoding a hydrophobic polypeptide of 149 amino acids. The polypeptide was rich in glutamine (20.0%), cysteine (10.0%), and methionine (6.9%). The cysteine content was higher than those of most other rice storage proteins. Messenger RNA of the 16.6-kDa prolamin was detected in seeds, but not in other aerial tissues.

• 社団法人日本農芸化学会の論文
• 1999-11-23

著者

• Tanaka K

  Tottori Univ. Tottori

• Masumura Takehiro

  Graduate School Of Life And Environmental Sci. Kyoto Prefectural Univ.

• Tanaka Kunisuke

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• MASUMURA Takehiro

  Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefect

• MITSUKAWA Norihiro

  Laboratory of Genetic Engineering, Faculty of Agriculture Kyoto Prefectural University

• KONISHI Ryoichi

  Laboratory of Genetic Engineering, Faculty of Agriculture Kyoto Prefectural University

• UCHIKI Masato

  Laboratory of Genetic Engineering, Faculty of Agriculture Kyoto Prefectural University

• Tanaka Kiyoshi

  Coll. Environ. Health Azabu Univ.

• Masumura T

  Biotechnology Res. Dep. Kyoto Prefectural Agriculture Forestry And Fisheries Technol. Center

• Uchiki Masato

  Laboratory Of Genetic Engineering Faculty Of Agriculture Kyoto Prefectural University

• Mitsukawa Norihiro

  Laboratory Of Genetic Engineering Faculty Of Agriculture

• Mitsukawa Norihiro

  Mitsui Plant Biotech.res.inst.

• Tanaka Kunisuke

  Laboratory Of Biochemistry College Of Agriculture Kyoto Prefectural University

• Masumura Takehiro

  Laboratory Of Genetic Engineering Graduate School Of Agriculture Kyoto Prefectural University

• Konishi Ryoichi

  Laboratory Of Genetic Engineering Faculty Of Agriculture

• Kikuchi Takao

  Coll. Environ. Health Azabu Univ.

• Mitsukawa Norihiro

  Forestry Research Institute Oji Paper Co. Ltd.:(present Address)toyota Central R&d Labs. Inc.

• Mitsukawa Norihiro

  Mitsui Plant Biotech. Res. Inst.

• 光川 典宏

  豊田中研

• Tanaka Kyoko

  Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

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