Improvement in the In Vivo Digestibility of Rice Protein by Alkali Extraction Is Due to Structural Changes in Prolamin/Protein Body-I Particle

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概要

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• Rice prolamin, constituting type-I protein body (PB-I), is indigestible and causes deterioration of rice protein nutritional quality. In this study, the in vivo digestibility of rice protein isolates was investigated by tracing their intraluminal transit in the gastrointestinal (GI) tract of rats by western blotting and by observing the structures excreted in the feces by electron microscopy. Two types of rice protein isolates, produced by alkali extraction (AE-RP) and by starch degradation (SD-RP), were compared. The protein patterns in the isolates were similar, but their digestion in the GI-tract showed striking differences. In the AE-RP group, 13-kDa prolamin (13P) quickly disappeared in the lower GI tract and was not excreted in the feces. By contrast, in the SD-RP group, 13P accumulated massively and nearly intact PB-Is were excreted. These results indicate that the in vivo digestibility of prolamin can be improved by alkali extraction through structural changes to it.

• 社団法人 日本農芸化学会の論文
• 2010-03-23

著者

• WATANABE REIKO

  Department of Nursing, Koseiren Nagaoka Chuo General Hospital

• KADOWAKI Motoni

  Department of Applied Biological Chemistry, Faculty of Agriculture, Graduate School of Science and T

• Masumura Takehiro

  Graduate School Of Life And Environmental Sci. Kyoto Prefectural Univ.

• Watanabe Reiko

  Dep. Of Health And Nutrition Univ. Of Niigata Prefecture

• Watanabe Reiko

  Department Of Genetics Research Institute Of Environmental Medicine Nagoya University

• KUMAGAI Takehisa

  Kameda Seika Co., Ltd.

• Kadowaki Motoni

  Dep. Of Applied Biological Chemistry Graduate School Of Sci. And Technol. Niigata Univ.

• Kadowaki Motoni

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Fujimura Shinobu

  Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Niigata 950-

• Watanabe Reiko

  Department Of Health And Nutrition University Of Niigata Prefecture

• Masumura Takehiro

  Graduate School Of Life And Environmental Science Kyoto Prefectural University

• KUBOTA Masatoshi

  Department of Applied Biological Chemistry, Graduate School of Science and Technology, Niigata Unive

• SAITO Yuhi

  Graduate School of Life and Environmental Science, Kyoto Prefectural University

• Saito Yuhi

  Lab. Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectural Uni

• Kumagai Takehisa

  Kameda Seika Co. Ltd.

• Fujimura Shinobu

  Department Of Applied Biological Chemistry Graduate School Of Science And Technology Niigata Univers

• Fujimura Shinobu

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University Niigata 950-218

• Kadowaki Motoni

  Department Of Applied Biological Chemistry Faculty Of Agriculture Graduate School Of Science And Tec

• Masumura Takehiro

  Laboratory Of Genetic Engineering Graduate School Of Life And Environmental Sciences Kyoto Prefectur

• Kumagai Takehisa

  Kameda Seika

• KADOWAKI Motoni

  Department of Applied Biological Chemistry

• KADOWAKI Motoni

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

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