A Rapid Increase in the Level of Binding Protein (BiP) is Accompanied bySynthesis and Degradation of Storage Proteins in Pumpkin Cotyledons
スポンサーリンク
概要
- 論文の詳細を見る
The binding protein (BiP) has been implicated in cotranslational folding of nascent polypeptides, and in the recognition and disposal of aberrant polypeptides. To elucidate the involvement of BiP in the biosynthesis of vacuolar proteins, we have characterized the protein in pumpkin cotyledons during seed maturation and seedling growth. Isolated microsomes from maturing pumpkin cotyledons contained a significant amount of BiP, protein-disulfide isomerase and calreticulin. We have purified a 70-kDa protein; sequences of the N-terminus and internal fragments of this protein exhibited a high identity to the sequence of soybean BiP. Immunoblot analysis with specific antibodies raised against the purified BiP showed that the amount of BiP in a cotyledon increased markedly at the middle stages and then decreased. The increase was accompanied by the synthesis of storage proteins and the development of the endoplasmic reticulum in the cotyledons at the middle stage of seed maturation. Most of these storage proteins degraded dramatically between 2 and 5 days after seed germination, and the degradation was also accompanied by a rapid increase in the level of BiP. Subcellular fractionation of the 4-day-old cotyledons showed a high accumulation of BiP in the endoplasmic reticulum. It is possible that BiP might be involved in the synthesis of seed storage proteins during maturation and in the synthesis of hydrolytic enzymes responsible for the degradation of the storage proteins during seed germination.
- 日本植物生理学会の論文
著者
-
NISHIMURA Mikio
Department of Cell Biology, National Institute for Basic Biology
-
Shimada Tomoo
Department Of Botany Graduate School Of Science Kyoto University
-
Nishimura Mikio
Department Of Cell Biology National Institute For Basic Biology
-
HIRAIWA Nagako
Dept. Cell Biol., Nat. Inst. Basic Biol.
-
Hara-Nishimura lkuko
Department of Cell Biology, National Institute for Basic Biology
-
Shimada Tomoo
Department Of Biology Faculty Of Science Kobe University
-
Shimada Tomoo
Dept. Cell Biol. Nat. Inst. Basic Biol.
-
Hatano Kyoko
Department Of Cell Biology National Institute For Basic Biology:department Of Natural Environment Sc
-
Hiraiwa Nagako
Department of cell Biology, National Institute for Basic Biology
-
Nishimura M
Dept. Cell Biol. Nat. Inst. Basic Biol.
-
Hiraiwa Nagako
Dept. Cell Biol. Nat. Inst. Basic Biol.
-
Hara-nishimura Lkuko
Department Of Cell Biology National Institute For Basic Biology:department Of Molecular Biomechanics
-
Hara-nishimura Lkuko
Department Of Cell Biology National Institute For Basic Biology:department Of Natural Environment Sc
-
Nishimura Mikio
National Inst. For Basic Biology Okazaki Jpn
関連論文
- Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
- Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells
- Cytosolic Aconitase Participates in the Glyoxylate Cycle in Etiolated Pumpkin Cotyledons : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM
- Spatial Patterns of Sucrose-Inducible and Polygalacturonic Acid-Inducible Expression of Genes That Encode Sporamin and β-Amylase in Sweet Potato : GENE STRUCTURE AND EXPRESSION
- Isolation of the Gene for EILP, an Elicitor-Inducible LRR Receptor-Like Protein, from Tobacco by Differential Display
- Molecular Cloning of a Defense-Response-Related Cytochrome P450 Gene from Tobacco
- The Involvement of Tonoplast Proton Pumps and Na^+(K^+)/H^+ Exchangers in the Change of Petal Color During Flower Opening of Morning Glory, Ipomoea tricolor cv. Heavenly Blue
- A Proteinase-Storing Body that Prepares for Cell Death or Stresses in the Epidermal Cells of Arabidopsis
- A NOVEL LARGE BODY THAT IS DERIVED FROM ENDOPLASMIC RETICULUM
- β-Glucosidase in the Indigo Plant : Intracellular Localization and Tissue Specific Expression in Leaves
- Heterologous Expression and Subcellular Localization of Pumpkin Seed Tonoplast Intrinsic Proteins (TIP) in Yeast Cells
- cDNA CLONING AND EXPRESSION OF PLASTIDAL CHAPERONIN 10(AT2)FROM Arabidopsis thaliana
- Detection and Characterization of a Vacuolar Protein(VP24) in Anthocyanin Producing Cells of Sweet Potato in Suspension Culture : PROTEINS, ENZYMES AND METABOLISM : STRUCTURE AND FUNCTION OF CELLS
- AtVAM3 is Required for Normal Specification of Idioblasts, Myrosin Cells
- An ER-Localized Form of PV72, a Seed-Specific Vacuolar Sorting Receptor, Interferes the Transport of an NPIR-Containing Proteinase in Arabidopsis Leaves
- Functional Differentiation of Peroxisomes Revealed by Expression Profiles of Peroxisomal Genes in Arabidopsis thaliana
- The ER Body, a Novel Endoplasmic Reticulum-Derived Structure in Arabidopsis
- A Vacuolar Sorting Receptor PV72 on the Membrane of Vesicles that Accumulate Precursors of Seed Storage Proteins (PAC Vesicles)
- Proteomic Analysis of Leaf Peroxisomal proteins in Greening Cotyledons of Arabidopsis thaliana
- Direct Interaction and Determination of Binding Domains among Peroxisomal Import Factors in Arabidopsis thaliana
- Distribution and Characterization of Peroxisomes in Arabidopsis by Visualization with GFP : Dynamic Morphology and Actin-Dependent Movement
- Activation of Arabidopsis Vacuolar Processing Enzyme by Self-Catalytic Removal of an Auto-Inhibitory Domain of the C-Terminal Propeptide
- Ped3p is a Peroxisomal ATP-Binding Cassette Transporter that might Supply Substrates for Fatty Acid β-Oxidation
- Developmental Analysis of a Putative ATP/ADP Carrier Protein Localized on Glyoxysomal Membranes During the Peroxisome Transition in Pumpkin Cotyledons
- Pumpkin Peroxisomal Ascorbate Peroxidase is Localized on Peroxisomal Membranes and Unknown Membranous Structures
- THE ANALYSIS OF THE TRANSPORT PATHWAY TO VACUOLES DIRECTLY FROM ER
- BINDING ANALYSIS OF A VACUOLAR SORTING RECEPTOR, PV72
- A NOVEL VACUOLAR MEMBRANE PROTEIN, MP73,IS TRANSPORTED TO VACUOLES VIA PAC VESICLES
- THE PROCESSING MECHANISM AND THE PHYSIOLOGICAL FUNCTION OF VEGETATIVE TYPE VACUOLAR PROCESSING ENZYME IN HIGHER PLANTS
- Distribution and characterization of peroxisomes in Arabidopsis thaliana by visualization with GFP
- Peroxisomal Localization of a Myosin XI Isoform in Arabidopsis thaliana
- Novel Glyoxysomal Protein Kinase, GPK1, Identified by Proteomic Analysis of Glyoxysomes in Etiolated Cotyledons of Arabidopsis thaliana
- Expression and Localization of a 36-kDa Peptide Derived from a 24-kDa Vacuolar Protein (VP24) Precursor in Anthocyanin-Producing Sweet Potato Cells in Suspension Culture
- Tissue and Intracellular Localization of Indican and the Purification and Characterization of Indican Synthase from Indigo Plants
- A Novel Isoenzyme of Ascorbate Peroxidase Localized on Glyoxysomal and Leaf Peroxisomal Membranes in Pumpkin : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
- Development of Microbody Membrane Proteins during the Transformation of Glyoxysomes to Leaf Peroxisomes in Pumpkin Cotyledons : MEMBRANES AND BIOENERGETICS : STRUCTURE AND FUNCTION OF CELLS
- Cloning and Sequencing of cDNA for Glycolate Oxidase from Pumpkin Cotyledons and Northern Blot Analysis
- Characterization of Intravacuolar Pigmented Structures in Anthocyanin-Containing Cells of Sweet Potato Suspension Cultures
- The Sequence and Expression of the γ-VPE Gene, One Member of a Family of Three Genes for Vacuolar Processing Enzymes in Arabidopsis thaliana : GENE STRUCTURE AND EXPRESSION
- Whole-body Cancer Screening Using ^F-FDG Novel Combined PET/CT Scanner
- Ammonium Ions Control Gametic Differentiation and Dedifferentiation in Chlamydomonas reinhardtii : GROWTH AND DEVELOPMENT : ENVIRONMENTAL AND STRESS RESPONSES
- Identification of an Allele of VAM3/SYP22 that Confers a Semi-dwarf Phenotype in Arabidopsis thaliana
- ISOLATION AND CHARACTERIZATION OF ARABIDOPSIS MUTANTS THAT HAVE DEFECTS IN THE PROCESSING OF SEED STORAGE PROTEINS
- THE LOCALIZATION OF PUMPKIN PV72,A SORTING RECEPTOR FOR VACUOLAR PROTEINS
- Characterization of Organelles in the Vacuolar-Sorting Pathway by Visualization with GFP in Tobacco BY-2 Cells
- Characterization of a putative vacuolar sorting receptor, PV72,in developing pumpkin seeds
- TRANSPORT PATHWAYS FOR SEED STORAGE PROTEINS TO THE VACUOLES OF HIGHER PLANTS
- ANALYSIS OF THE VACUOLAR TARGETING SIGNAL OF PUMPKIN PRO2S ALBUMIN
- MOLECULAR CHARACTERIZATION OF ARABIDOPSIS CO-CHAPERONINS.
- MULTIPLE FUNCTIONAL PROTEINS ARE PRODUCED BY CLEAVING ASN-GLN BONDS OF A SINGLE PRECURSOR BY VACUOLAR PROCESSING ENZYME
- A Pumpkin 72-kDa Membrane Protein of Precursor-Accumulating Vesicles Has Characteristics of a Vacuolar Sorting Receptor
- A Rapid Increase in the Level of Binding Protein (BiP) is Accompanied bySynthesis and Degradation of Storage Proteins in Pumpkin Cotyledons
- A NOVEL MEMBRANE PROTEIN, PMP38,IS LOCALIZED ON PLANT GLYOXYSOMAL MEMBRANES
- INTRACELLULAR TRANSPORT OF A LEAF PEROXISOMAL 27-kDa PROTEIN FROM Arabidopsis thaliana
- Microbody Defective Mutants of Arabidopsis
- MOLECULAR MECHANISM FOR TARGETING OF PLANT CATALASE TO MICROBODIES
- Hydroxypyruvate Reductase with a Carboxy-Terminal Targeting Signal to Microbodies is Expressed in Arabidopsis
- cDNA Cloning and Expression of a Gene for Isocitrate Lyase in Pumpkin Cotyledons
- Functional Transformation of Microbodies in Higher Plant Cells
- Functional Classification of Arabidopsis Peroxisome Biogenesis Factors Proposed from Analyses of Knockdown Mutants
- Oligomeric Proteins Containing N-Terminal Targeting Signals are Imported into Peroxisomes in Transgenic Arabidopsis
- Accumulation of a Fusion Protein Containing 2S Albumin Induces Novel Vesicles in Vegetative Cells of Arabidopsis
- ASCORBATE PEROXIDASE THAT IS LOCALIZED IN MICROBODY MEMBRANES
- CHARACTERIZATION OF VEGETATIVE-TYPE VACUOLAR PROCESSING ENZYME of Arabidopsis
- ARABIDOPSIS ped2 MUTANT ACCUMULATES PRECURSOR WITH HIGH MOLECULAR MASS FOR PTS2-TYPE PEROXISOMAL PROTEINS
- PURIFICATION AND CHARACTERIZATION OF A PEROXISOMAL SHORT-CHAIN ACYL-COA OXIDASE FROM PUMPKIN
- MOLECULAR CHARACTERIZATION OF ACYL-CoA OXIDASE IN PUMPKIN
- ARABIDOPSIS ped1 MUTANT THAT HAS DEFECT IN THIOLASE GENE
- Glyoxysomal Malate Dehydrogenase in Pumpkin : Cloning of a cDNA and Functional Analysis of Its Presequence
- Changes in Targeting Efficiencies of Proteins to Plant Microbodies Caused by Amino Acid Substitutions in the Carboxy-terminal Tripeptide
- Cryptochromes and Phytochromes Synergistically Regulate Arabidopsis Root Greening under Blue Light
- Reduction to below Threshold Levels of Glycolate Oxidase Activities in Transgenic Tobacco Enhances Photoinhibition during Irradiation
- Behavior of Vacuoles during Microspore and Pollen Development in Arabidopsis thaliana
- Development of Enzymes of the Glyoxylate Cycle during Senescence of Pumpkin Cotyledons
- Glyoxylate Cycle Enzymes in Peroxisomes Isolated from Petals of Pumpkin (Cucurbita sp.) during Senescence
- Arabidopsis MUTANTS THAT HAVE DEFECTS ON GLYOXYSOMAL FUNCTIONS
- A Vacuolar Processing Enzyme in Maturing and Germinating Seeds : Its Distribution and Associated Changes during Development
- CHARACTERIZATION OF A PROTEIN COMPONENT, DV100, OF THE DENSE VESICLES FROM MATURING PUMPKIN SEEDS
- Small GTP-Binding Proteins are Associated with the Vesicles That are Targeted to Vacuoles in Developing Pumpkin Cotyledons : GROWTH AND DEVELTOPMENT : PROTEINS, ENZYMES AND METABOLISM
- Ectopic Expression of an Esterase, Which is a Candidate for the Unidentified Plant Cutinase, Causes Cuticular Defects in Arabidopsis thaliana
- Suppression of Peroxisome Biogenesis Factor 10 Reduces Cuticular Wax Accumulation by Disrupting the ER Network in Arabidopsis thaliana
- Constitutive and Inducible ER Bodies of Arabidopsis thaliana Accumulate Distinct β-Glucosidases
- Arabidopsis VPS35, a Retromer Component, is Required for Vacuolar Protein Sorting and Involved in Plant Growth and Leaf Senescence
- RECOGNITION OF THE N-TERMINAL TARGETING SIGNAL OF GLYOXYSOMAL CITRATE SYNTHASE PRECURSOR IN TRANSGENIC ARABIDOPSIS
- cDNA CLONING AND EXPRESSION OF A GENE FOR THE ENZYMES OF β-OXIDATION CYCLE IN PUMPKIN COTYLEDONS
- THE N-TERMINAL REGION OF GLYOXYSOMAL MALATE DEHYDROGENASE FROM PUMPKIN FUNCTION IN TARGETING TO MICROBODIES
- MAG4/Atp115 is a Golgi-Localized Tethering Factor that Mediates Efficient Anterograde Transport in Arabidopsis
- Plant Catalase is Imported into Peroxisomes by Pex5p but is Distinct from Typical PTS1 Import
- Vacuolar Processing Enzyme of Soybean That Converts Proproteins to the Corresponding Mature Forms : GROWTH AND DEVELTOPMENT : PROTEINS, ENZYMES AND METABOLISM
- Antagonistic Jacalin-Related Lectins Regulate the Size of ER Body-Type β-Glucosidase Complexes in Arabidopsis thaliana
- Proteomic Analysis of Highly Purified Peroxisomes from Etiolated Soybean Cotyledons
- Vacuolar SNAREs Function in the Formation of the Leaf Vascular Network by Regulating Auxin Distribution
- cDNA CLONING OF CHAPERONIN10 FROMARABIDOPSIS THALIANA AND ITS EXPRESSION
- Frontiers of Research on Organelle Differentiation
- Seeing Is Believing : On the Use of Image Databases for Visually Exploring Plant Organelle Dynamics
- Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
- cDNA Cloning of Thylakoid-Bound Ascorbate Peroxidase in Pumpkin and Its Characterization
- The Plant Organelles Database 2 (PODB2) : An Updated Resource Containing Movie Data of Plant Organelle Dynamics
- A Defect of Peroxisomal Membrane Protein 38 Causes Enlargement of Peroxisomes
- Loss of XRN4 Function Can Trigger Cosuppression in a Sequence-Dependent Manner