Using promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
- 論文の詳細を見る
By application of the high-efficiency loss of heterozygosity (HELOH) method for disrupting genes in diploid sake yeast (Kotaka et al., Appl. Microbiol. Biotechnol., 82, 387-395 (2009)), we constructed, from a heterozygous integrant, a homozygous diploid that overexpresses the alcohol acetyltransferase gene ATF2 from the SED1 promoter, without the need for sporulation and mating. Under the conditions of sake brewing, the homozygous integrant produced 1.4 times more isoamyl acetate than the parental, heterozygous strain. Furthermore, the homozygous integrant was more genetically stable than the heterozygous recombinant. Thus, the HELOH method can produce homozygous, recombinant sake yeast that is ready to be grown on an industrial scale using the well-established procedures of sake brewing. The HELOH method, therefore, facilitates genetic modification of this rarely sporulating diploid yeast strain while maintaining those characteristics required for industrial applications.
- 社団法人日本生物工学会の論文
著者
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Kotaka Atsushi
Research Institute, Gekkeikan Sake Co. Ltd.
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Sahara Hiroshi
Research Institute, Gekkeikan Sake Co. Ltd.
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Kondo Akihiko
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University
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Ueda Mitsuyoshi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Hata Yoji
Research Institute, Gekkeikan Sake Co. Ltd.
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Hata Yoji
Research Institute Gekkeikan Sake Co. Ltd.
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Sahara Hiroshi
Research Institute Gekkeikan Sake Co. Ltd.
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Kotaka Atsushi
Research Institute Gekkeikan Sake Co. Ltd.
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Hata Yoji
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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Ueda Mitsuyoshi
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Kondo Akihiko
Department Of Applied Chemistry Kyushu Institute Of Technology
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Kondo Akihiko
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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