Deletion Analysis of the Catalase-Encoding Gene (catB) Promoter from Aspergillus oryzae
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概要
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The catalase-encoding gene (catB) is expressed strongly in Aspergillus oryzae. To identify the transcription regulatory elements involved in strong expression, we did promoter deletion analysis using β-glucuronidase (GUS) as a reporter and an electrophoretic gel mobility shift assay (EMSA) systematically. The deletion 200-bp sequence from −1,000 to −800 in the 1,400-bp catB promoter caused a drastic decrease in GUS activity. In addition, EMSA implicated a 45-bp element from −1,000 to −956 containing cis-elements. According to detailed promoter deletion analysis, a region from −1,000 to −975, which contains putative heat shock element (HSE) and the CCAAT-box, was involved in strong expression.
- 社団法人 日本農芸化学会の論文
著者
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Hata Yoji
Research Institute, Gekkeikan Sake Co. Ltd.
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Sano Motoaki
Gene Regulation Group Molecular And Cell Biology National Inst. Of Advanced Industrial Sci. And Tech
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Abe Yasuhisa
Research Institute For Fundamental Physics Kyoto University : Centre De Recherches Nucleaires
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HISADA HIROMOTO
Research Institute, Gekkeikan Sake Co. Ltd.
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MACHIDA Masayuki
Gene Regulation Group, Molecular and Cell Biology, National Institute of Advanced Industrial Science
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