Biochemical Analysis of the Yeast Proteinase Inhibitor (I^C) Homolog I^Ch and Its Comparison with I^C

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概要

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• Carboxypeptidase Y (CPY) inhibitor (IC) and its homologous protein (ICh) are thought to be members of the phosphatidylethanolamine-binding protein (PEBP) family of Saccharomyces cerevisiae. The biochemical characterization of IC and its inhibition mode toward CPY were recently reported, but ICh has not been characterized. The molecular mass of ICh was determined to be 22,033.7. The N-terminal Met1 was cleaved and the amino group of Ser2 was acetylated. ICh is folded as a monomeric β-protein and is devoid of disulfide bonds. It has no inhibitory activity toward CPY, and it does not form a complex with CPY. ICh was exclusively expressed in the early log phase, whereas IC was expressed in the logarithmic and stationary phase. The intracellular localization of ICh was different from that of IC. These findings provide insights into the physiological functions of ICh.

• 社団法人 日本農芸化学会の論文
• 2007-02-23

著者

• Ueda Mitsuyoshi

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• MIMA Joji

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Nagayama Mitsuru

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Mima Joji

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Ueda Mitsuyoshi

  Division Of Applied Life Science Graduate School Of Agriculture Kyoto University

• FUKADA Hiroaki

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• KATO Michiko

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Kato Michiko

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Fukada Hiroaki

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Nagayama Mitsuru

  Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.

• Nagayama Mitsuru

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Fukuda Hiroaki

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

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