Two-Dimensional Protein Separation by the HPLC System with a Monolithic Column
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概要
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A two-dimensional high-performance liquid chromatography (2D-HPLC) system for protein separation was developed using an ion-exchange column in the first dimension and a reversed-phase monolithic column in the second dimension. The system demonstrated efficient separation of proteins in comparison with conventional systems. For proteomic analysis, proteins extracted from the cell surface of the yeast were separated by 2D-HPLC and evaluated.
- 2012-03-23
著者
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Ueda Mitsuyoshi
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Morisaka Hironobu
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Morisaka Hironobu
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Kobayashi Kengo
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Kirino Aya
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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