Production of MBP(Maltose Binding Protein)-GroES Fusion Protein and Utilization to Stimulate GroEL-Mediated Protein Refolding
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概要
- 論文の詳細を見る
GroES, the co-chaperonin of Escherichia coli, was overproduced and purified using the maltose binding protein(MBP)fusion vector. The MBP-GroES fusion protein was expressed in a soluble form in E. eoli and purified to homogeneity using amylose resin in a single step. The MBP-GroES fusion protein was found to form a heptamer, reduce the ATPase activity of chaperonin GroEL and stimulate GroEL-mediated protein refolding, to a simihr extent as the wild-type GroES. Furthermore, the MBP-GroES fusion protein bound GroEL, and this complex was recovered easily from the refolding mixture by amylose resin. These results suggest that the combination of the MBP-GroES fusion protein and GroEL is effective for the construction of a repeated refolding system of proteins.
- 社団法人日本生物工学会の論文
- 1998-01-25
著者
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Kondo Akihiko
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University
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ISHII Yoshiyuki
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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Ishii Yoshiyuki
Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Ishii Yoshiyuki
東京大学 農生命科研究
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Ishii Yoshiyuki
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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OGAWA Hiroaki
Department of Thermonuclear Fusion Research,Naka Fusion Research Establishment,Japan Atomic Energy R
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Ishii Yoshiyuki
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Ogawa H
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Kato Yasuhiko
Department of Anesthesiology, Osaka Dental University
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MURAKAMI KAORU
Department of Applied Chemistry, Faculty of Enggineering, Kyushu Institute of Technology
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Kondo Akihiko
Department Of Bioactive Molecules National Institute Of Infectious Diseases
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Murakami Kaoru
Department Of Applied Biological Science Fukuyama University
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Murakami Kaoru
Department Of Applied Chemistry Faculty Of Enggineering Kyushu Institute Of Technology
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I.-ogawa Hiroaki
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Ogawa Hiroaki
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Kato Yasuhiko
Department Of Anesthesiology Osaka Dental University
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Kondo Akihiko
Department Of Applied Chemistry Kyushu Institute Of Technology
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Kondo Akihiko
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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