Changes in mRNA Expression in Mouse Postnatal Cochlea by Differential Display Method

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概要

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• We compared mRNA expression by mRNA differential display method in postnatal day 11 (P11), P13 and adult C3H/HeJ mouse cochlea. Forty-seven bands were differentially displayed on polyacrylamide gel when 27 patterns of PCR primer sets were used, and 24 of them showed a remarkable increase within only two days (P11 and P13). DNA sequences of the bands were analyzed for homology to known genes using the BLAST search. Most of the clones were identical to sequences of functions unknown. However, one of the clones showing an increase of mRNA expression between P11 and P13 was identified as mouse TIS7 which is known to markedly increase during differentiation of PC-12 cells to neurons by NGF-treatment. TIS7 expression may be involved in differentiation of neuronal progenitor cells to spiral ganglion cells by the initial sound input. The comparison among P11, P13 and adult mouse cochlear mRNA expressions investigated the genes involved in the various growing stages of the postnatal cochlea.

• 社団法人　日本実験動物学会の論文
• 2002-10-01

著者

• 吉川 泰弘

  東京大学 大学院農学生命科学研究科実験動物学教室

• ISHII Yoshiyuki

  Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University

• KYUWA Shigeru

  Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University

• YOSHIKAWA Yasuhiro

  Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University

• KIKKAWA Yoshiaki

  Department of Bioindustry, Tokyo University of Agriculture

• Ishii Yoshiyuki

  Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• Ishii Yoshiyuki

  東京大学 農生命科研究

• KAMIYA Kazusaku

  Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University

• Ishii Yoshiyuki

  Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of

• Yoshikawa Yasuhiro

  Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• Kyuwa S

  Univ. Tokyo Tokyo Jpn

• Kyuwa Shigeru

  Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of

• Yoshikawa Y

  Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• Yoshikawa Yasuhiro

  Department Of Biomedical Science Graduate School Of Agricultural And Life Science University Of Toky

• Ishii Y

  Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• Ishii Yoshiyuki

  Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology

• Kyuwa Shigeru

  Department Of Animal Pathology Institute Of Medical Science University Of Tokyo

• Kamiya Kazusaku

  Department Of Biomedical Science Graduate School Of Agricultural And Life Science University Of Toky

• Kikkawa Y

  Department Of Laboratory Animal Science The Tokyo Metropolitan Institute Of Medical Science

• 吉川 泰弘

  Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of

• Kyuwa Shigeru

  Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences University Of Tok

• Kyuwa Shigeru

  Center For Experimental Medicine Institute Of Medical Science The University Of Tokyo

• Yoshikawa Yasuhiro

  Department Of Biomedical Science Faculty Of Agriculture The University Of Tokyo

• 吉川 泰弘

  Department Of Biomedical Science Faculty Of Agriculture The University Of Tokyo

• Kikkawa Y

  Tokyo Metropolitan Inst. Medical Sci. Tokyo Jpn

• 吉川 泰弘

  Dep. Of Biomedical Sci. Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• Kikkawa Yoshiaki

  Department Of Bioindustry Tokyo University Of Agriculture

• Kitagawa Yota

  Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of

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