Characterization of Embryonic Stem-Like Cell Lines Derived from Embryoid Bodies
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概要
- 論文の詳細を見る
Embryoid bodies (EB) were formed by TT2 embryonic stem (ES) cells in vitro. ES-like cell lines (ESLC) were established by culturing cells obtained by disaggregation of EB at 4, 8 and 20 days after culture, and designated ESLC4, ESLC8 and ESLC20, respectively. Flow cytometric analysis indicated that the cell surface expression of Lex on ESLC was less than that of original TT2 ES cells, but the expression of L-CAM was comparable. After suspension culture, all of the ESLC cells formed cystic EB in vitro. In addition, some ESLC4- and ESLC8-derived EB showed signs of beating. Although coat color chimeras were able to be produced with ESCL4 at a lower rate than parental ES cells, the cells did not contribute to germ line cells in chimeras. These results suggest that the ESLC had less pluripotent than parental ES cells and also that EB formation is not useful in obtaining pluripotent cells.
- 社団法人 日本実験動物学会の論文
著者
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KYUWA Shigeru
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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Sato Eimei
Department of Reproductive and Developmental Biology, Institute of Medical Science, The University o
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Sato E
Tohoku Univ. Sendai Jpn
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Kyuwa Shigeru
Department Of Animal Pathology Institute Of Medical Science University Of Tokyo
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Kyuwa Shigeru
Department Of Reproductive And Developmental Biology Institute Of Medical Science University Of Toky
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XIAO Yingda
Department of Reproductive and Developmental Biology, Institute of Medical Science, University of To
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TOYODA Yutaka
Department of Reproductive and Developmental Biology, Institute of Medical Science, University of To
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Xiao Yingda
Department Of Reproductive And Developmental Biology Institute Of Medical Science University Of Toky
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Toyoda Yutaka
Department Of Reproductive And Developmental Biology Institute Of Medical Science The University Of
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Toyoda Yutaka
Department Of Reproductive And Developmental Biology Institute Of Medical Science University Of Toky
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Sato Eimei
Department Of Reproductive And Developmental Biology Institute Of Medical Science University Of Toky
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Sato Eimei
Department Of Reproductive And Developmental Biology Institute Of Medical Science The University Of
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Toyoda Yutaka
Department of Animal Pathology, Institute of Medical Science, University of Tokyo
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