Role of Cumulus Oophorus in Increasing Efficiency of Fertilization in Mouse Oocytes <I>In Vitro</I>
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概要
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The functional role of the cumulus oophorus during fertilization was examined. It was found that the sperm concentration necessary for successful fertilization was markedly different between cumulus-intact and cumulus-free oocytes; only 5 sperm/μl were required for the maxi-mum level of fertilization of cumulus-intact oocytes, while 150 sperm/μl were needed for cumu-lus-free oocytes. Fertilization rate did not increase by co-culturing cumulus-free oocytes with detached cumulus cells in the fertilization medium. On the other hand, supplementing the fertili-zation medium with soluble factor(s) vortexed out of cumulus-oocyte complexes significantly improved the rate of fertilization (56% in the treated group vs 36% in controls; P<0.05) when spermatozoa were preincubated in BSA-supplemented medium. No significant effect, however, was observed when spermatozoa were preincubated in protein-free medium. These results strongly suggest that soluble factor(s) sustained in cumulus-oocyte complexes promote fertilization, and the integrity of the three dimensional structure of the cumulus oophorus is important for efficient sperm-egg interaction.
著者
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KAMIO Kae
Department of Applied Biology, Nihon University
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Toyoda Yutaka
Department Of Reproductive And Developmental Biology Institute Of Medical Science The University Of
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Sato Eimei
Department Of Reproductive And Developmental Biology Institute Of Medical Science The University Of
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Imahie Hiroshi
Safety Research Laboratory Research Division Mitsubishi Tanabe Pharma Corporation
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Takahashi Yuji
Department Of Dermatology Course Of Integrated Medicine Graduate School Of Medicine Osaka University
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AZUMA Sadahiro
Department of Animal Pathology, Institute of Medical Science,The University of Tokyo
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AZUMA Sadahiro
Department of Reproductive and Developmental Biology, Institute of Medical Science, The University of Tokyo
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Toyoda Yutaka
Department of Animal Pathology, Institute of Medical Science, University of Tokyo
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KAMIO Kae
Department of Reproductive and Developmental Biology, Institute of Medical Science, The University of Tokyo
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