Effects of Cadmium in Vitro on Contractile and Relaxant Responses of Isolated Rat Aortas
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概要
- 論文の詳細を見る
Objective:Cadmium is known to affect the vascular tone of isolated blood vessels in vitro and the arterial pressure of rats in vivo. However, the mechanisms of cadmium actions on the vascular system have not been clarified. To elucidate the actions of cadmium on vascular tonus, effects of cadmium on vasocontractile and vasorelaxant responses in vitro were investigated using aortic strips isolated from rats. Methods: Aortic strips isolated from male Wistar rats were incubated with CdCl_2 (10 μM) for 24 hr, washed with fresh CdCl_2-free medium, and then used for measurement of isometric tension and Western blot analysis of eNOS (endothelial nitric oxide synthase) and iNOS (inducible nitric oxide synthase). Results: In the aortas pretreated with cadmium in vitro, the contractile response to phenylephrine was significantly higher than that in the control aortic strips pretreated with a vehicle. The sodium nitroprusside-induced relaxing response was significantly higher in the aortic strips pretreated with cadmium for 24 hr, compared with that in the control pretreated with a vehicle. The isoproterenolinduced relaxing response was also significantly higher in the cadmium-accumulated nortic strips. In vitro cadmium treatment slightly but not significantly increased the acetylcholine-induced relaxation of the aortic strips. Cadmium treatment induced expression of iNOS and significantly increased expression of eNOS in the aortic strips, while it did not affect expression of β-actin. Conclusions: Cadmium treatment in vitro augmented the nui adreaoceptor-mediated contractile response, even though eNOS and iNOS were upregulated by cadmium treatment. NO-induced and β-adrenoceptor-mediated relaxing responses were also augmented by cadmium treatment. These results suggest that both vasocontractile and vasorelaxing responses are augmented in cadmiumaccumulated aortas.
- 日本衛生学会の論文
著者
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Takahashi Yuji
Department of Neurology, Graduate School of Medicine, University of Tokyo
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WAKABAYASHI Ichiro
Department of Hygiene & Preventive Medicine
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KOIZUMI Naoko
Department of Marine Bioresources Chemistry, Faculty of Fisheries, Hokkaido University
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Koizumi N
Department Of Public Health Hyogo College Of Medicine
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Koizumi Naoko
Department Of Marine Bioresources Chemistry Faculty Of Fisheries Hokkaido University
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POTESER Michael
Department of Hygiene &Preventive Medicine, Yamagata University School of Medicine
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MASUI Hidehisa
Department of Public Health, Hyogo College of Medicine
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Wakabayashi Ichiro
Department Of Environmental And Preventive Medicine Hyogo College Of Medicine
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Wakabayashi Ichiro
Department Of Hygiene &preventive Medicine Yamagata University School Of Medicine
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Masui Hidehisa
Department Of Public Health Hyogo College Of Medicine
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Masui Hidehisa
Department Of Hygiene Hyogo College Of Medicine
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Takahashi Yuji
Department Of Internal Medicine Division Of Cardiology And Memorial Heart Center Iwate Medical Unive
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Poteser Michael
Department Of Hygiene &preventive Medicine Yamagata University School Of Medicine
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Takahashi Yuji
Dep. Of Environmental And Preventive Medicine Hyogo Coll. Of Medicine Jpn
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Takahashi Yuji
Department Of Environmental And Preventive Medicine Hyogo College Of Medicine
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Takahashi Yuji
Department Of Dermatology Course Of Integrated Medicine Graduate School Of Medicine Osaka University
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