Typing of X Chromosomes Bearing Tabby Allele in Mouse Preimplantation of a Microsatellite Marker

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概要

• 論文の詳細を見る

• Tabby (Ta) is a semidominat allele of the locus on the mouse X chromosome, which causes a characteristic coat pattern and developmental defects in endocrinic glands. To establish a method for identifying the X chromosome bearing the Ta allele in early preimplantation embryos, we performed PCR amplification of an X chromosomal microsatellite marker locus from preimplantation embryos obtained from mating between XO female and XY male mice. The microsatellite marker locus was shown to be polymorphic between X chromosomes bearing the Ta and wild-type alleles. The amplification of the marker locus from early preimplantation embryos demonstrated that the Ta locus can be correctly typed by this method from embryos as little as 2 cells.

• 社団法人　日本実験動物学会の論文
• 1996-10-01

著者

• TOYODA Yutaka

  Department of Reproductive and Developmental Biology, Institute of Medical Science, University of To

• KUNIEDA Tetsuo

  Faculty of Agriculture, Okayama University

• Kunieda Tetsuo

  Faculty Of Agriculture Okayama University

• Toyoda Yutaka

  Department Of Reproductive And Developmental Biology University Of Tokyo

• Hattori Masahira

  Laboratory for Molecular Medicine, Institute of Medical Science, the University of Tokyo

• Hattori M

  Univ. Tokyo Tokyo

• Hattori Masahira

  Laboratory For Molecular Medicine Institute Of Medical Science The University Of Tokyo

• VALDIVIA Ruby

  Laboratory of Molecular Biology, Faculty of Odontology, University of Chile, Santiago

• Valdivia Ruby

  Laboratory Of Molecular Biology Faculty Of Odontology University Of Chile Santiago

• Toyoda Yutaka

  Department Of Reproductive And Developmental Biology Institute Of Medical Science The University Of

• Toyoda Yutaka

  Department of Animal Pathology, Institute of Medical Science, University of Tokyo

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