Germ-Line Contribution of Embryonic Stem Cells in Chimeric Mice: Influence of Karyotype and In Vitro Differentiation Ability

スポンサーリンク

概要

• 論文の詳細を見る

• The effect of the karyotype and the ability to differentiate in vitro upon germ-line transmission by A3-1 embryonic stem (ES) cells in chimeric mice were examined. Germ-line transmission was confirmed in ES cells exhibiting 38% and more of the normal karyotype, but no chimeric mice and/or germ-line transmitters were observed regardless of the karyotype when the cystic embryoid body (CEB) was formed on day 8 and later in the suspension culture. Germ-line transmission of the ES cells was not significantly influenced by formation of the simple embryoid body (SEB). Germ-line transmitters were preferentially observed in chimeras when the ES cell contribution to coat color was markedly increased, but this contribution to coat color varied regardless of the karyotype or in vitro differentiation ability. These results suggest that A3-1 ES cells which exhibit CEB at 7 days after suspension culture and approximately 40% of normal karyotype are capable of germ-line transmission in chimeric mice.

• 社団法人　日本実験動物学会の論文

著者

• Harada Koichiro

  Department Of Physiological Chemistry And Metabolism Molecular Cell Biology Universtiy Of Tokyo Grad

• Yazaki Y

  Department Of Gene Diagnostics And Therapeutics International Medical Center Of Japan

• YAZAKI Yoshio

  Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo

• Yamori Yukio

  日本大学 内科2

• Terauchi Y

  Univ. Tokyo Tokyo Jpn

• Kodama Tatsuhiko

  Laboratory For System Biology And Medicine Rcast The University Of Tokyo

• Kamada N

  Chugai Pharmaceutical Co. Ltd. Shizuoka

• Suzuki Hiroshi

  Department Of Hepatology Toranomon Hospital

• Yazaki Yoshio

  Third Department Of Internal Medicine Faculty Of Medicine University Of Tokyo

• KADOWAKI TAKASHI

  Third Department of Internal Medicine,Faculty of Medicine,University of Tokyo

• Yazaki Yoshio

  Department Of Cardiovascular Medicine Graduate School Of Medicine University Of Tokyo

• Yazaki Yoshio

  Department Of Gene Diagnostics And Therapeutics International Medical Center Of Japan

• Terauchi Yasuo

  Third Department Of Internal Medicine Faculty Of Medicine University Of Tokyo

• Kodama Tatsuhiko

  Department Of Molecular Biology And Medicine Research Center For Advanced Science And Technology Uni

• Kodama Tatsuhiko

  Research Center Of Advanced Science And Technology University Of Tokyo

• SUZUKI Hiroshi

  Laboratory of Molecular Genetics and Embryology, CSK Research Park Inc.

• KAMADA Nobuo

  Laboratory of Molecular Genetics and Embryology, CSK Research Park Inc.

• UEDA Otoya

  Laboratory of Molecular Genetics and Embryology, CSK Research Park Inc.

• JISHAGE Kouichi

  Laboratory of Molecular Genetics and Embryology, CSK Research Park Inc.

• KURIHARA Yukiko

  Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo

• KURIHARA Hiroki

  Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo

• AZUMA Sadahiro

  Department of Reproductive and Developmental Biology, Institute of Medical Science, The University o

• KODAMA Tatsuhiko

  Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo

• TOYODA Yutaka

  The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veteri

• Takahashi Kiyoshi

  Fuji Bio Co. Ltd.

• Kodama Tatsuhiko

  Third Department Of Internal Medicine Faculty Of Medicine University Of Tokyo

• Toyoda Y

  Univ. Tokyo Tokyo Jpn

• Toyoda Y

  Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn

• Toyoda Yutaka

  The Research Center For Protozoan Molecular Immunology Obihiro University Of Agriculture And Veterin

• Azuma S

  Mitsubishi Kasei Inst. Life Sci. Tokyo Jpn

• Kurihara Hiroki

  Department Of Internal Medicine Graduate School Of Medicine The University Of Tokyo

• Kurihara Hiroki

  Third Department Of Internal Medicine Faculty Of Medicine University Of Tokyo

• Takahashi K

  Fuji Bio Co. Ltd.

• Takahashi K

  Research Institute For Applied Mechanics Kyushu University

• Jishage K

  Laboratory Of Molecular Genetics And Embryology Csk Research Park Inc.

• Kurihara Yukiko

  Department Of Physiological Chemistry And Metabolism University Of Tokyo Graduate School Of Medicine

• Ueda Otoya

  Laboratory Of Molecular Genetics And Embryology Csk Research Park Inc.

• Ueda O

  Fuji-gotemba Research Laboratories Chugai Pharmaceutical Co.

• Sano Hiroyuki

  Chugai Pharmaceutical Co. Ltd.

• Suzuki H

  Department Of Hepatology Toranomon Hospital

• Toyoda Yutaka

  Research Unit For Functional Genomics National Research Center For Protozoan Diseases Obihiro Univer

• Kurihara H

  Department Of Physiological Chemistry And Metabolism University Of Tokyo Graduate School Of Medicine

• Yazaki Yoshio

  Third Department Of Internal Medicine Faculty Of Medicine The University Of Tokyo.

• Kadowaki Takashi

  Third Department Of Internal Medicine Faculty Of Medicine University Of Tokyo

• Harada Koichiro

  Third Department Of Internal Medicine University Of Tokyo

• AZUMA Sadahiro

  Department of Animal Pathology, Institute of Medical Science,The University of Tokyo

関連論文

• The Japanese Society of Hypertension Committee for Guidelines for the Management of Hypertension
• PE-335 A Novel Muscle-restricted Coiled-coil Protein, MURC, Induces Cardiac Dysfunction Accompanied with an Altered Gene Expression Profile(Heart failure, basic(03)(M),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation
• PE-335 A Novel Muscle-restricted Coiled-coil Protein, MURC, Induces Cardiac Dysfunction Accompanied with an Altered Gene Expression Profile(Heart failure, basic(03)(M),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation
• Clinical Practice Guidebook for Diagnosis and Treatment of Chronic Kidney Disease
• Prevalence of chronic kidney disease (CKD) in the Japanese general population predicted by the MDRD equation modified by a Japanese coefficient
• Sirolimus-Eluting Stents vs Bare Metal Stents for Coronary Intervention in Japanese Patients With Renal Failure on Hemodialysis
• Simple sampling strategy for measuring inulin renal clearance
• Estimation of glomerular filtration rate by the MDRD study equation modified for Japanese patients with chronic kidney disease
• Nifedipine Retard Was as Effective as Angiotensin Converting Enzyme Inhibitors in Preventing Cardiac Events in High-Risk Hypertensive Patients with Diabetes and Coronary Artery Disease : The Japan Multicenter Investigation for Cardiovascular Diseases-B (J
• Successful Isolation of a Chromosome 1 Region Affecting Blood Pressure, Stroke, and Glucose Tolerance In the Stroke-Prone Spontaneously Hypertensive Rat
• OJ-011 Association of Obesity Related Factors with Plasma B-type Natriuretic Peptide Levels in General Japanese Population(OJ02,Obesity/SAS (H),Oral Presentation (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
• Impact of Metabolic Syndrome Components on the Incidence of Cardiovascular Disease in a General Urban Japanese Population : The Suita Study
• FRS-076 A link between LDL Receptor (LDLR) Gene Mutation and Clinical Manifestations in Familial Hypercholesterolemia(FRS16,Basic Mechanisms of Lipid Disorders and Metabolic Syndrome (H),Featured Research Session (English),The 73rd Annual Scientific Meeti
• PJ-898 Predicting high risk patients of coronary artery disease in heterozygous familial hypercholesterolemia from clinical features(Lipid disorders(05)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
• Frameshift Mutations of the hMSH6 Gene in Human Leukemia Cell Lines
• Rapid progression of chronic myelomonocytic leukemia following diaminodiphenyl sulphone treatment for dermatitis herpetiformis
• Subcellular localization of the MEN, MLI/MEN and truncated MLL proteins expressed in leukemic cells carrying the t(11;19)(q23;p13.1) translocation
• c-Cbl Is Inducibly Tyrosine-phosphorylated by Epidermal Growth Factor Stimulation in Fibroblasts, and Constitutively Tyrosine-phosphorylated and Associated with v-Src in v-src-transformed Fibroblasts
• Tyrosine Phosphorylation of the Proto-oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT-7
• 1077 EFFECTS OF OUABAIN AND DIETARY SALT ON CONTRACTION AND RELAXATION OF ARTERIAL SMOOTH MUSCLE OF Na^+ /H^+ EXCHANGER OVEREXPRESSED TRANSGENIC MICE
• -0156- NOREPINEPHRINE-INDUCED CONTRACTION AND EFFECTS OF VERAPAMIL AND RYANODINE IN ISOLATED AORTIC RINGS FROM TRANSGENIC MICE OVEREXPRESSING Na^+/H^+ EXCHANGER(PROCEEDINGS OF THE 59th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY)
• Biphasic Activation of Two Mitogen-activated Protein Kinases during the Cell Cycle in Mammalian Cells.
• Cellular and Molecular Bases for the Immunopathology of the Myocardial Cell Damage Involved in Acute Viral Myocarditis with Special Reference to Dilated Cardiomyopathy : MOLECULAR ANALYSIS OF THE PATHOPHYSIOLOGY OF CARDIOMYPATHY
• 8)DISTINCTIVE PATTERNS OF MYOSIN ISOZYMES IN THE CONDUCTION SYSTEM AND ABNORMAL MUSCLE BUNDLES OF HUMAN HEARTS : THE 7th CONFERENCE ON CARDIOMYOPATHY
• A NEW METHOD OF IMAGES OF MYOCARDIAL INFARCTION WITH LABELLED MONOCLONAL ANTIBODY SPECIFIC FOR VENTRICULAR MYOSIN HEAVY CHAIN : Nuclear Cardiology (I) : FREE COMMUNICATIONS (I) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATIO
• Applications of Anticardiac Myosin Monoclonal Antibodies in the Diagnosis of Heart Disease : PANEL DISCUSSION ON PROGRESS IN NONINVASIVE CARDIAC DIAGNOSIS
• FRS-102 Event rates of Japanese patients with atherothrombosis : Results from the REduction of Atherothrombosis for Continued Health (REACH) Registry(Clinical Frontiers in Atherosclerosis(IHD),Featured Research Session,The 72nd Annual Scientific Meeting o
• COMPARISON OF THE ENZYMATIC CHARACTERISTICS AND SYNTHESIS OF CARDIAC MYOSINS FROM RIGHT AND LEFT VENTRICLES : IVth Auditorium : PROCEEDINGS OF THE 40TH ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
• Difference between Fluvastatin and Simvastatin on the eNOS Gene Expression (Cardiovascular Pharmacology, Basic/Clinical 2 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• 1 Beyond lowering blood pressure effect of nifedipine retard : Nifedipine retard prevents hospitalization for angina pectoris better than angiotensin-converting enzyme inhibitors in hypertensive Japanese patients with a history of myocardial infarction (J
• Signal Transduction Mechanism of Insulin and Insulin-Like Growth Factor-1
• Unstable Angina and Non-ST Elevation Acute Coronary Syndrome : Epidemiology and Current Management in Japan (Japan Multicenter Investigation for Cardiovascular Disease-D (JMIC-D) Committee)
• Genetic Analysis of Dilated Cardiomyopathy : HLA and immunoglobulin genes may confer susceptibility : MOLECULAR ANALYSIS OF THE PATHOPHYSIOLOGY OF CARDIOMYPATHY
• 1066 INVOLVEMENT OF ENDOTHELIN -1 IN MECHANICAL STRETCH-INDUCED CARDIAC HYPERTROPHY
• PHENOTYPIC MODULATION OF SMOOTH MUSCLE CELLS DURING DEVELOPMENT OF HUMAN CORONARY ATHEROSCLEROSIS AS DETERMINED BY MYOSIN HEAVY CHAIN EXPRESSION
• Endothelial Responses of the Aorta from Adrenomedullin Transgenic Mice and Knockout Mice
• -447- CARDIAC HYPERTROPHY AND MYOSIN ISOZYME COMPOSITION IN SPONTENEOUS HYPERTENSIVE RATS : EFFECTS OF β-BLOCKERS
• Lack of Sex-Specific Effects on the Association between Angiotensin-Converting Enzyme Gene Polymorphism and Hypertension in Japanese
• 0802 ANGIOTENSINII IS NOT NECESSARY FOR MECHANICAL STRETCH-INDUCED ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN CARDIAC MYOCYTES OF AT1 KNOCKOUT MOUSE
• The Multiple Roles of Macrophage Scavenger Receptors (MSR) in vivo: Resistance to Atherosclerosis and Susceptibility to Infection in MSR Knockout Mice
• TRANSCRIPTIONAL REGULATION OF A NON-MUSCLE TYPEMYOSIN HEAVY CHAIN (SMemb) IN SMOOTH MUSCLE CELLS
• Germ-Line Contribution of Embryonic Stem Cells in Chimeric Mice: Influence of Karyotype and In Vitro Differentiation Ability
• Vasopressin as a Possible Contributor to Hypertension : SYMPOSIUM ON BLOOD PRESSURE AND VASOACTIVE SUBSTANCES
• EFFECTS OF DIETARY SODIUM ON BRAIN ANGIOTENSIN II RECEPTORS IN SPONTANEOUSLY HYPERTENSIVE RATS
• The Importance of Vasopressin in the Mechanism Maintaining Hypertension in the Rat : THE 10th CONFERENCE ON THE PATHOGENESIS OF HYPERTENSION
• OE-287 Comprehensive investigation of quantitative trait loci for blood pressure and associated metabolic traits in the spontaneous hypertensive rat strains(Genetics/Genetically Engineered Models 2 (H) : OE36)(Oral Presentation (English))
• Evaluation of Gene-Gene Interaction for Hypertension and Associated Metabolic Traits in Reciprocal Crossbreeds Derived from Chromosome-1 Congenic Rats
• Angiotensin II Type I Receptor Antagonist Prevents Renal Damage in Aged Transgenic Mice Overexpressing Endothelin-1
• DEVELOPMENT OF A NOVEL TARGETED GENE DELIVERY SYSTEM USING BACTERIAL BIO-MAGNETIC PARTICLES (MAGNETOSOMES)
• Forced Expression of MyoD Suppresses the Proliferation of Smooth Muscle Cells
• IS013 Endothelin-1 promotes cranial/cardiac neural crest development as a potential mediator of epithelial-mesenchymal interaction
• The Mechanism of Insulin-induced Signal Transduction Mediated by the Insulin Receptor Substrate Family
• 0863 ANALYSIS OF FOCAL ADHESION RELATED TYROSINE KINASE IN CARDIAC MYOCYTE STIMULATED BY VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF)
• Molecular Mechanism of Hypertrophied Failing Heart : Abnormalities of the diastolic properties and contractility : CLINICAL EVALUATION OF CARDIAC PERFORMANCE
• ISOZYMIC CHANGES IN MYOSIN OF HUMAN VENTRICULAR MYOCARDIUM INDUCED BY OVERLOAD. IMMUNOHISTOCHEMICAL STUDY USING MONOCLONAL ANTIBODY : Myocardial Biochemistry : PROCEEDINGS OF THE 50th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• -332-A NEW METHOD FOR INFARCT SIZING : IMAGING OF ACUTE MYOCARDIAL INFARCTION WITH In-111 LABELED ANTIMYOSIN HEAVY CHAIN MONOCLONAL ANTIBODY
• Molecular Mechanism of Cardiac Hypertrophy : 53th Annual Scientific Session of the Japanese Circulation Society
• STUDIES ON MYOSIN ISOENZYMES IN THE ATRIUM : Myocardial metabolism . Biochemistry : 46th Annual Scientific Meeting, Japanese Circulation Society
• ELECTROPHORETIC ALTERATION OF S-1 AND LIGHT CHAIN 1 OF RABBIT CARDIAC VENTRICULAR MYOSIN INDUCED BY THYROXINE-TREATMENT AND PRESSURE-OVERLOAD : Myocardial metabolism . Biochemistry : 46th Annual Scientific Meeting, Japanese Circulation Society
• -0957- ISOLATION AND CHARACTERIZATION OF MOUSE SMOOTH MUSCLE MYOSIN HEAVY CHAIN(SM1/2)GENE PROMOTER(PROCEEDINGS OF THE 59th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY)
• GENE EXPRESSION OF THE RABBIT VASCULAR PLATELET DERIVED GROWTH FACTOR SYSTEM IN THE ARTERIAL WALL DURING VASCULAR REMODELING AND IN CULTURED SMOOTH MUSCLE CELLS
• Targeted Disruption of Adrenomedullin and αCGRP Genes Reveals Their Distinct Biological Roles
• Protective effect of adrenomedullin against cardiac hypertrophy and renal damage
• Left Cervical Aortic Arch With Aortic Coarctation and Saccular Aneurysm
• IS037 Both Ca^2+/calmodulin-dependent kinase II and calcineurin play critical roles in cardiac hypertrophy
• Responses of Blood Pressure and Catecholamine Metabolism to High Salt Loading in Endothelin-1 Knockout Mice
• IS017 Growth hormone protects cardiac myocytes against H_2O_2-induced apoptosis through activating extracelluar signal-regulated kinase
• P311 ANALYSIS OF VENTRICULAR CONTRACTION IN THE ENDOTHELIN-1 GENE KNOCK OUT MOUSE
• PJ-243 A Novel Mutation in the Autosomal Recessive Hypercholesterolemia Gene Leads to Hypercholesterolemia(Lipid disorders-3 (H) PJ41,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• Resistin Gene Polymorphism Positively Associated with the Metabolic Syndrome in Japanese Men(Gene Therapy/Genetic Engineering (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• Evidence of Cell-Mediated Cardiac Myocyte Injury Involved in the Heart Failure of a Patient With Progressive Systemic Sclerosis
• Perforin-Secreting Killer Cell Infiltration in the Aortic Tissue of Patients With Atherosclerotic Aortic Aneurysm
• Perforin-Positive Leukemic Cell Infiltration in the Heart of a patient with T-Cell Prolymphocytic Leukemia
• Expression of Myosin Isozymes during the Developmental Stage and Their Redistribution Induced by Pressure Overload : SYMPOSIUM ON LONG-TERM MYOCARDIAL CHANGES IN HEART DISEASES : 49th Annual Scientific Session of the Japanese Circulation Society
• Evaluation of Selected polymorphisms of the Mendelian Hypertensive Disease Genes in the Japanese Population
• -P204- ISOZYMIC CHANGES OF MYOSIN IN CARDIAC HYPERTROPHY
• The Myosin Gene Switching in Human Cardiac Hypertrophy : 53th Annual Scientific Session oh the Japanese Circulation Society
• -0072-THE MOLECULAR MECHANISM OF STRETCH INDUCED CARDIAC HYPERTROPHY
• -0068-TRANSCRIPTIONAL REGULATION OF THE HUMANN VENTRICULAR MYOSIN ALKALI LIGHT CHAIN GENE
• -0067-THE MOLECULAR MECHANISM OF ANGIOTENSIN II INDUCED C-FOS GENE EXPRESSION ON CARDIOMYOCYTES
• -0066-ANGIOTENSION II INDUCES HYPERTROPHY AND ONCOGENE EXPRESSION IN CULTURED RAT HEART MYOCYTE
• -137-REEXPRESSION OF FETAL TYPE mRNAS IN THE VENTRICLE OF CARDIOMYOPATHIC SYRIAN HAMSTER : Cardiomyopathy : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 53th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• Molecular Analysis of Cardiac Hypertrophy
• -P205- RAPID INDUCTION OF SPECIFIC mRNAs BY CARDIAC PRESSURE OVERLOAD
• Distribution of Cardiac Myosin Isozymes in Cardiomyopathy : Immunohistochemical and Gene Analysis : SYMPOSIUM ON CURRENT STATUS OF DILATED CARDIOMYOPATHY : 50th Annual Scientific Session of the Japanese Circulation Society
• -358-ABNORMAL EXPRESSION OF ONE OF β TYPE MYOSIN ISOZYMES IN VENTRICLES OF PATIENTS WITH DILATED CARDIOMYOPATHY
• 6)THE MOLECULAR BASIS FOR GENE EXPRESSION OF MYOSIN ISOZYMES IN HUMAN HEART : THE 8th CONFERENCE ON CARIDIOMYOPATHY
• ISOLATION AND CHARACTERLIZATION OF HUMAN CARDIAC MYOSIN LIGHT CHAIN 1 GENE : Cardiac Metabolism : FREE COMMUNICATIONS (II) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• evidence-based practice guideline for the treatment of chronic kidney disease
• Mechanisms of Cardiac Hypertrophy and Injury : Possible Role of Protein Kinase C Activation : 54th Annual Scientific Session of the Japanese Circulation Society
• OE-293 Genetic Analysis of Metabolic Syndrome in Rats(Genetics/Genetically Engineered Models 2 (H) : OE36)(Oral Presentation (English))
• -0530-MESSENGER RNA LEVELS OF GUANINE NUCLEOTIDE BINDING PROTEINS ARE REDUCED IN THE VENTRICLES OF CARDIOMYOPATHIC HAMSTERS
• ISOLATION AND CHARACTERIZATION OF TWO β-TYPE CARDIAC MYOSIN IN THE CANINE ATRIUM
• EFFECTS OF PRESSURE OVERLOAD ON THE MYOSIN ISOZYMES IN HUMAN VENTRICULAR AND SPECIALIZED MYOCARDIUM : Conduction System : FREE COMMUNICATIONS (IV) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• HETEROGENEITY OF β-TYPE MYOSIN ISOZYMES IN HUMAN HEART : REGULATIONAL MECHANISMS IN THEIR EXPRESSION : Cardiac Metabolism : FREE COMMUNICATIONS (II) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• The combined impact of blood pressure category and glucose abnormality on the incidence of cardiovascular diseases in a Japanese urban cohort : the Suita Study
• Comparison of a simple and a standard method for inulin renal clearance
• OE-357 GATA-4 is required for cardiac morphogenesis through transactivation of N-cadherin gene(Development and Differentiation 2 (H) : OE45)(Oral Presentation (English))
• OE-356 Novel Transmembrane Type Class VI Semaphorin, Sema6D Regulates Cardiac Morphogenesis Through PlexinA1(Development and Differentiation 2 (H) : OE45)(Oral Presentation (English))
• 'INVERSES' OF VIRASORO OPERATORS(Finite groups and related topics)

もっと見る 閉じる

スポンサーリンク