Asparagine-linked Glycosylation of the Rat Leukemia Inhibitory Factor Expressed by Simian COS7 Cells
スポンサーリンク
概要
- 論文の詳細を見る
The leukemia inhibitory factor (LIF) is a secretory glycoprotein and a pluripotent growth factor which acts in diverse cell systems. LIF has been reported to be heavily glycosylated. In this paper, we examine the transient expression of rat LIF (rLIF) in COS7 cells and its glycosylation by a PNGaseF treatment and lectin blot. rLIF expression in COS7 cells resulted in seven molecular species being produced with zero to six N-glycosyl moieties. Mutated rLIF proteins with substitutions at the seven possible N-glycosy-lation sites were also expressed. An analysis of the molecular weight of the mutated rLIF confirmed the six N-glycosylation sites. Bioassays of mouse leukemia cell lines were performed to analyze the contribution of the glycosyl moieties to their functions. We found that the glycosyl moieties at each of the N-glycosylation sites were not essential to their function of the protein, but the reduced functions to promote the proliferation of DA-1a cells that had been observed for some mutants suggests a biochemical role for the in vitro function.
- 社団法人日本農芸化学会の論文
- 1998-07-23
著者
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KYUWA Shigeru
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University
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Ogawa Tomoya
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive
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Ogawa T
Animal Resource Science/veterinary Medical Science University Of Tokyo
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Sato Eimei
Department of Reproductive and Developmental Biology, Institute of Medical Science, The University o
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SATO Eiichiro
Department of Metallurgical Engineering, Faculty of Engineering, Chiba Institute of Technology
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Kyuwa S
Univ. Tokyo Tokyo Jpn
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Shiota Kunio
Laboratory Of Cellular Biochemistry Veterinary Medical Science/animal Resource Science The Universit
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Shiota Kunio
Department Of Veterinary Physiology Veterinary Medical Science The University Of Tokyo
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Shiota Kunio
Department Of Veterinary Biochemistry Faculty Of Agriculture The University Of Tokyo
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Kawamura S
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences The University Of
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AIKAWA Junichi
Laboratory of Synthetic Cellular Chemistry, RIKEN (the Institute of Physical and Chemical Research)
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SASAI Ken
Department of Animal Resource Science, Graduate School of Agricultural Life Science, the University
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Kyuwa Shigeru
Department Of Animal Pathology Institute Of Medical Science University Of Tokyo
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Shiota K
Univ. Tokyo Tokyo Jpn
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Kyuwa Shigeru
Department Of Biomedical Science Graduate School Of Agricultural And Life Sciences University Of Tok
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Kyuwa Shigeru
Department Of Reproductive And Developmental Biology Institute Of Medical Science The University Of
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Kyuwa Shigeru
Center For Experimental Medicine Institute Of Medical Science The University Of Tokyo
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Sasai Ken
Laboratory Of Cellular Biochemistry The University Of Tokyo
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Sato Eiichiro
Department Of Animal Resource Science Graduate School Of Agricultural Life Science The University Of
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Aikawa J
Laboratory Of Synthetic Cellular Chemistry Riken (the Institute Of Physical And Chemical Research)
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Aikawa Jun-ichi
Department Of Biotechnology Graduate School Of Agriculture And Life Sciences The University Of Tokyo
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Ogawa Tomoya
Laboratory Of Cellular Biochemistry Animal Resource Sciences/veterinary Medical Sciences The Univers
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Shiota K
Animal Resource Science/veterinary Medical Science University Of Tokyo
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Sato Eimei
Department Of Reproductive And Developmental Biology Institute Of Medical Science The University Of
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Shiota Kunio
Laboratory Of Cellular Biochemistry Animal Resource Sciences/veterinary Medical Sciences The Univers
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Ogawa Tomoya
Laboratory Of Cellular Biochemistry Animal Resource Science/veterinary Medical Science The Universit
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Shiota Kunio
Department of Cellular Biochemistry
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Ogawa Tomoya
Laboratory for Physics and Technology of Crystals, 2-9-21-604 Shin-sayama, Sayama, Saitama 350-1331, Japan
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