FAB CID-MS/MS of In-Source Fragment Ions as a Means to Differentiate Linkage Isomers of Branched Oligosaccharides : Le^a and Le^x Type Glycoconjugates

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概要

• 論文の詳細を見る

• Here we report that FAB CID-MS/MS, choosing an ion originating from the in-source degradation prior to the full acceleration of the molecule-related ion as the precursor, is an excellent means to differentiate linkage isomers. FAB CID-MS/MS performed in this way results in a product ion spectrum, which is enriched with the fragmentation information, and which may thus indicate the position of the newly formed OH group already produced in the ion source. Linkage knowledge may be classified into one of three categories as described later. This method was applied to acidic Lewis-type glycoconjugates —putative ligands for selectins [L. L. W. Cooling, D.-S. Zhang, and T. A. W. Koerner, Trends in Glycosci. Glycotech., 9, 191 (1997)]— and their neutral analogs to differentiate linkage isomers by mass spectrometry. With these glycoconjugates FAB CID-MS/MS having [M+H]+s as the precursor ions is a good means to distinguish the linkage isomers only in simple cases, but not in general cases. By using ions originating from in-source degradation prior to the full acceleration of the molecule-related ion as the precursor, we were able to differentiate between Lea and Lex.

• 日本質量分析学会の論文
• 1998-02-01

著者

• Ogawa Tomoya

  Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive

• NIWA Haruki

  Department of Applied Physics and Chemistry, The University of Electro-Communications

• OHASHI Mamoru

  Department of Materials Science, Kanagawa University

• 丹羽 治樹

  電気通信大学

• Niwa Haruki

  Department Of Applied Physics And Chemistry The University Of Electro-communications

• Ogawa Tomoya

  Laboratory Of Synthetic Cellulary Chemistry The Institute Of Physical And Chemical Research (riken)

• OHASHI Yoko

  Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research (R

• II Tadashi

  Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research (R

• KUBOTA Masayuki

  Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research (R

• NUNOMURA Shigeki

  Tokyo Pharmaceutical Research Laboratories, Nissin Food Products Co., Ltd.

• NAGAI Yoshitaka

  Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research (R

• Nagai Y

  Glycobioloyg Research Frontier Research Program The Institute Of Physical And Chemical Research (rik

• Nagai Y

  Mitsubishi Kasei Institute Of Life Sciences

• Ii Tadashi

  Glycobiology Research Froniter Research Program The Institute Of Physical And Chemical Research (rik

• Ohashi Y

  Niigata Univ. School Of Medicine Niigata

• Ohashi Yoko

  Glycobiology Research Frontier Research Program The Institute Of Physical And Chemical Research (rik

• Ohashi Mamoru

  Food And Drug Administration Center For Drugs And Biologics Division Of Drug Chemistry

• Ogawa T

  Osaka Univ. Dental School Osaka

• Ohashi Mamoru

  Department Of Applied Physics And Chemistry The University Of Electro-communications

• Kubota Masayuki

  Department Of Applied Physics And Chemistry The University Of Electro-communications

• Ogawa Tomoya

  Laboratory Of Cellular Biochemistry Animal Resource Sciences/veterinary Medical Sciences The Univers

• Ogawa Tomoya

  Laboratory Of Cellular Biochemistry Animal Resource Science/veterinary Medical Science The Universit

• Nunomura Shigeki

  Tokyo Research Institute Nissin Food Products Corporation

• Ogawa Tomoya

  Laboratory for Physics and Technology of Crystals, 2-9-21-604 Shin-sayama, Sayama, Saitama 350-1331, Japan

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