FAB-CID Mass Spectrometry of Imidazolone Derivatives Related to the Chromophore of Aequorea Green Fluorescent Protein
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概要
- 論文の詳細を見る
Positive and negative ion fast atom bombardment tandem mass spectrometry (FAB-CID MS/MS) has established the fragmentation pattern of the 18 imidazolone derivatives which are related to the structure of the GFP (green fluorescent protein) chromophore in the jellyfish Aequorea bioluminescence. Ring cleavage of the C5-N1 and C2-N3 bonds is characteristics of these compounds, but for NH derivatives cleavage of the methylidene group as well as the C4-C5 and C2-N3 bonds also occurred in the positive ion mode, while ring cleavage of the C2-N3 and C4-C5 bonds occurred commonly in the negative ion mode. On the basis of the CID-MS/MS (collision induced dissociation tandem mass spectrometry) the structures of protonated molecules produced upon FAB are discussed.When the structure of the fragment ion has a p-quinonoid moiety, distinct peaks due to radical anion species such as [M-H-CH3]-· are observed in their CID-MS/MS of parent anions [M-H]-, which seems to be characteristics of the conjugated p-hydroxyphenyl structures.
- 日本質量分析学会の論文
- 1998-02-01
著者
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KUBOTA Masayuki
Department of Pediatric Surgery, Niigata University Graduate School of Medical and Dental Sciences
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平野 誉
電気通信大学 量子・物質工学科
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HIRANO Takashi
Department of Applied Physics and Chemistry, The University of Electro-Communications
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NIWA Haruki
Department of Applied Physics and Chemistry, The University of Electro-Communications
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OHASHI Mamoru
Department of Materials Science, Kanagawa University
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丹羽 治樹
電気通信大学
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Niwa Haruki
Department Of Applied Physics And Chemistry The University Of Electro-communications
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HIRAKI Toshikazu
Central Customs Laboratory, Ministry of Finance
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Ohashi Mamoru
Food And Drug Administration Center For Drugs And Biologics Division Of Drug Chemistry
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Ohashi Mamoru
Department Of Applied Physics And Chemistry The University Of Electro-communications
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Kubota Masayuki
Department Of Applied Physics And Chemistry The University Of Electro-communications
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Kubota Masayuki
Departmanets Of Pediatric Surgery Graduate School Of Medical And Dental Sciences Niigata University
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Hirano Takashi
Department Of Applied Physics And Chemistry The University Of Electro-communications
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Hirano Takashi
Department Of Surgery Tokyo Metropolitan Health And Medical Treatment Corporation Ebara Hospital
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