Composition Analysis of Polar Lipids in Halobacteria with Mass Spectrometry

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概要

• 論文の詳細を見る

• The structures of the polar lipids in halobacteria including their mutants were determined with ESI-MS and FAB-MS. The composition-analyses with ESI-MS gave the results fairly close to these with TLC analyses. The percentages of the composite polar lipids by the mass spectrometric analyses of Halobacterium salinarum S-9 were 20, 77, and 5% for glycolipid sulfate (S-TGD), methyl ester of phosphatidyl glycerophosphate (PGP-OMe), and phosphatidylglycerol (PG), respectively. The MS/MS analysis in the FAB-MS provided an analyzing tool for the structural elucidation of the functional groups in the lipids. Newly proposed methylation in the phosphate was investigated with stable isotope experiments. We found that the Australian halobacteria, Halorubrum sp. aus-1, mainly consist of an isolated glycolipid sulfate with two sugar parts of glucopyranoses. In relationship with the lipid-composition analysis, the chemotaxonomy of halobacteria was discussed. The methylation of the lipids was proved to be essentially independent of that in the photosensory protein of halobacteria on the basis of the wide distribution of the methylated lipids.

• 日本質量分析学会の論文
• 2004-12-01

著者

• TSUJIMOTO Kazuo

  School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST)

• Mizukami Taku

  School Of Material Science Jaist

• MIYABAYASHI Keiko

  School of Materials Science, Japan Advanced Institute of Science and Technology

• OHASHI Mamoru

  Department of Materials Science, Kanagawa University

• Naito Y

  Japan Advanced Inst. Sci. And Technol. Ishikawa Jpn

• Tsujimoto K

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Tsujimoto Kazuo

  School Of Material Sciences Japan Advanced Inst. Of Sci. And Technol.

• Mizukami T

  Jaist Ishikawa

• Ohashi Mamoru

  Food And Drug Administration Center For Drugs And Biologics Division Of Drug Chemistry

• Miyabayashi Keiko

  School Of Material Science Jaist (hokuriku)

• NOGUCHI Yuuki

  School of Materials Science, Japan Advanced Institute of Science and Technology

• HAYASHI Akio

  School of Materials Science, Japan Advanced Institute of Science and Technology

• NAITO Yasuhide

  Institute of Free Electron Laser, Graduate School of Engineering

• Noguchi Yuuki

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Naito Yasuhide

  Institute Of Free Electron Laser Graduate School Of Engineering Osaka University

• Ohashi Mamoru

  Department Of Applied Physics And Chemistry The University Of Electro-communications

• Hayashi Akio

  School Of Material Sciences Japan Advanced Institute Of Science And Technology

• Hayashi Akio

  School Of Materials Science Japan Advanced Institute Of Science And Technology

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