Characterization of [1-^<13>C]-Specifically Labeled Amino Acids Using ESIMS/MS in the Selected Reaction Monitoring Mode
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概要
- 論文の詳細を見る
ESIMS/MS was employed for the analyses of 1-13CO-labeled amino acids. Conventionally, in order to estimate the 13C-content, the intensity of molecule-related ion of the sample has been compared against the natural compound. However, this method is incompetent for specifying the position of the 13C-labeling, hence is unable to distinguish the specifically 1-13C-labeled compound from otherwise-(generally or non-specifically) labeled amino acids. With the intention of distinguishing 1-13CO-labeled amino acids from generally labeled and non-labeled molecules we utilized ESIMS/MS in the selected reaction monitoring mode, choosing the protonated molecules as the precursor ion and specifying the product ion generated by a 47 u- (instead of 46 u-) elimination from the precursor ion for the labeled samples. By this new method, characterization of solely 1-13CO-labeled amino acids has been successfully conducted, enabling their distinction from otherwise-labeled compounds.
- 日本質量分析学会の論文
- 2005-12-01
著者
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Maki Shojiro
Department Of Applied Physics And Chemistry The University Of Electrocommunications
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Maki Shojiro
Department Of Applied Physics And Chemistry The University Of Electro-communications
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平野 誉
電気通信大学 量子・物質工学科
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HIRANO Takashi
Department of Applied Physics and Chemistry, The University of Electro-Communications
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NIWA Haruki
Department of Applied Physics and Chemistry, The University of Electro-Communications
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丹羽 治樹
電気通信大学
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Niwa Haruki
Department Of Applied Physics And Chemistry The University Of Electro-communications
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OHASHI Yoko
Department of Obstetrics and Gynecology, Hiroshima University
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HATASE Hiroshi
Department of Applied Physics and Chemistry, The University of Electro-Communications
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NAKAMURA Mitsuhiro
Department of Applied Physics and Chemistry, The University of Electro-Communications
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Ohashi Yoko
Center For Instrumental Analysis The Univ. Of Electro-communications
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Ohashi Yoko
Department Of Applied Physics And Chemistry The University Of Electro-communications
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Hatase Hiroshi
Department Of Applied Physics And Chemistry The University Of Electro-communications
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Ohmiya Y
Center For Instrumental Analysis The Univ. Of Electro-communications
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Hirano Takashi
Department Of Applied Physics And Chemistry The University Of Electro-communications
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Nakamura Mitsuhiro
Department Of Applied Physics And Chemistry The University Of Electro-communications
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Hirano Takashi
Department Of Surgery Tokyo Metropolitan Health And Medical Treatment Corporation Ebara Hospital
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