An Interspecies Comparison of Placental Gangliosides
スポンサーリンク
概要
- 論文の詳細を見る
The major ganglioside compositions of placental tissues from several species (rat, sea lion, porpoise, cow and horse) were compared. The samples were extracted with chloroform/methanol/water and the glycosphingolipid fractions were isolated through sequential treatment by peracetylation with pyridine/acetic anhydride, Florisil column chromatography, and deacetylation with sodium hydroxide. Gangliosides were isolated using DEAE Sephadex A-25 column chromatography. Their structures were identified by silica-gel thin-layer chromatography (TLC) and TLC/immunostaining using eight monoclonal antibodies that specifically recognize GM3, GM2, GD3, GD1a and IV3NeuAc-nLc4Cer (SPG). The major ganglioside in each placenta was GM3, comprising 50-80% of the total gangliosides. GM3 containing N-acetylneuraminic acid (NeuAc) was the major placental ganglioside in the sea lion and horse, whereas GM3 containing N-glycolylneuraminic acid (NeuGc) was the major ganglioside in the porpoise. In rat and cow placentas, the expression levels of both types of GM3 were equal. GD3 was detected in all five species. SPG was detected in them all except the rat. TLC/immunostaining detected GM2 (NeuAc) in the sea lion and cow, and GD1a (NeuAc, NeuAc) in the sea lion. We previously reported that the major gangliosides present in the rat placenta were GM3 and GD3. Therefore, GM3 and GD3 was commonly expressed in the placenta of six animal species examined.
- 日本繁殖生物学会の論文
著者
-
Shiota Kunio
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive
-
Ogawa Tomoya
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The Unive
-
TOBAYAMA Teruo
Kamogawa Sea World
-
TAI Tadashi
Department of Tumor,Immunology and Clinical Genetics,Tokyo Metropolitan Institute of Medical Science
-
SASAKI Nobuo
Laboratory of Veterinary Surgery, Faculty of Agriculture, University of Tokyo
-
Itonori S
Department Of Chemistry Faculty Of Liberal Arts And Education Shiga University
-
YOSHIOKA Motoi
Laboratory of Fish Culture, Faculty of Bioresources, Mie University
-
ITONORI Saki
Laboratory of Cellular Biochemistry, Animal Resource Science/Veterinary Medical Science, The Univers
-
FUKURO Mitsunari
Laboratory of Cellular Biochemistry, Animal Resource Science/Veterinary Medical Science, The Univers
関連論文
- Molecular Cloning of Equine Preprorelaxin cDNA
- Resistance to 5-aza-2'-deoxycytidine in Genic Regions Compared to Non-genic Repetitive Sequences
- EGGS RELEASED BY THE NOTOTHENIID FISH TREMATOMUS BERNACCHII BOULENGER IN CAPTIVITY (Eleventh Symposium on Polar Biology)
- Growth of nototheniid fish, Trematomus bernacchii and Pagothenia borchgrevinki reared in aquarium
- Rearing of Antarctic marine organisms in Japan (extended abstract)
- Trehalose May Prevent Postsurgical Adhesions in a Rabbit Model of Hysterotomy
- Accumulation of cholesterol and G_ ganglioside in cells cultured in the presence of progesterone:an implication for the basic defect in Niemann-Pick disease typc C
- Increased levels of GM_2 ganglioside in fibroblasts from a patient with juvenile Niemann - Pick disease type C
- Asparagine-linked Glycosylation of the Rat Leukemia Inhibitory Factor Expressed by Simian COS7 Cells
- Maxillofacial reconstruction using custom-made artificial bones fabricated by inkjet printing technology
- Tailor-made tricalcium phosphate bone implant directly fabricated by a three-dimensional ink-jet printer
- FAB CID-MS/MS of In-Source Fragment Ions as a Means to Differentiate Linkage Isomers of Branched Oligosaccharides : Le^a and Le^x Type Glycoconjugates
- A New Approach to the Characterization of Sulfated and Sialyl Lewis-Type Glycosphingolipids Using Positive-Ion FABMS, ESIMS, and CID-MS/MS
- Fast Atom Bombardment and Electrospray Ionization Tandem Mass Spectrometry of Sulfated Lewis_X Trisaccharides
- Classification into a Novel Mollu-Series of Neutral Glycosphingolipids from the Lamp Shell, Lingula unguis¶
- Ganglioside GT1b in Rat Brain Binds to p58,a Brain-Specific Sodium-Dependent Inorganic Phosphate Cotransporter:Expression Cloning with a Specific Monoclonal Antibody to Ganglioside GT1b-Binding Protein
- Differential distribution of ganglioside GM1 and sulfatide during the development of Xenoqus
- Evidence for Direct Binding of Intracellularly Distributed Ganglioside GM2 to Isolated Vimentin Intermediate Filaments in Normal and Tay-Sachs Disease Human Fibroblasts
- Functions of the N-Glycans of Rat Leukemia Inhibitory Factor Expressed in Chinese Hamster Ovary Cells
- Evaluation of the Role of N-linked Oligosaccharides in Rat Placental Lactogen Action by Site-Directed Mutagenesis
- Detection of Novel Molecules Recognized by Anti-Placental Lactogen Antibody in Rat Amniotic Fluid
- Site-Directed Mutagenesis of Recombinant Equine Chorionic Gonadotropin/Luteinizing Hormone: Differential Role of Oligosaccharides in Luteinizing Hormone-and Follicle-Stimulating Hormone-Like Activities
- Comparative external morphology of cetacean spermatozoa
- Neonatal Exposure to Diethylstilbestrol Alters Expression of DNA Methyltransferases and Methylation of Genomic DNA in the Mouse Uterus
- Neonatal Exposure to Diethylstilbestrol Alters the Expression of DNA Methyltransferases and Methylation of Genomic DNA in the Epididymis of Mice
- Generation and Characterization of a Monoclonal Antibody Recognizing a Fetal Brain Enriched O-Linked Sialoglycoprotein, FOG100
- Phosphonoglycolipids in Marine Crustacean : Structural Characterization of Two Novel Phosphonocerebrosides, from the Crab, Erimacrus isenbeckii
- Structural Characterization of a Novel Series of Fucolipids from the Marine Annelid, Pseudopotamilla occelata
- Biocompatibility of two different types of extracellular matrix in bioartificial endocrine pancreas in dogs: a preliminary report
- Expression of Sialylparagloboside in a Case of Liposarcoma: Aberrant Glycosylation in Tumors Arising in Adipose Tissues
- Bone regeneration within a tailor-made tricalcium phosphate bone implant with both horizontal and vertical cylindrical holes transplanted into the skull of dogs
- Globotriaosyl Ceramide and Globoside as Major Glycolipid Components of Fibroblasts in Scirrhous Gastric Carcinoma Tissues
- Polysialogangliosides Expressed by Amelanotic Melanoma : A Possible Explanation for the Poor Response to Anti-monosialoganglioside Antibody 202 in a Patient with Melanoma
- Production of a Recombinant Single-Chain Variable-Fragment (scFv) Antibody against Sulfoglycolipid
- An Interspecies Comparison of Placental Gangliosides
- An Interspecies Comparison of Placental Gangliosides
- Serum Cortisol Levels in Captive Killer Whale and Bottlenose Dolphin
- Biological Activities of Tethered Equine Chorionic Gonadotropin (eCG) and Its Deglycosylated Mutants
- Molecular Cloning of Equine Preprorelaxin cDNA
- Relationship between Sexual Maturity and Serum and Testis Testosterone Concentrations in Short-finned Pilot Whales Globicephala macrorhynchus
- SI-4 Immunohistochemical Analysis of Glycosphingolipids in the Central Nervous System Using Specific Monoclonal Antibodies
- Release of Anterior Pituitary Hormones by Trypsin-pretreated Anterior Pituitary Cells
- Current status of cetaceans and other marine mammals in the North Pacific, with a review of advanced research activities on cetacean biology in Japan
- Apoptosis of Rat Uterine NK Cells In Vitro
- Prolactin Receptor mRNA Expression in Fetal Rat Brain
- Phosphocholine-Containing Glycosyl Inositol-Phosphoceramides from Trichoderma viride Induce Defense Responses in Cultured Rice Cells
- Nucleotide Sequence of eCG α-Subunit cDNA and Its Expression in the Equine Placenta
- Accumulation of GM2 Ganglioside in Niemann-Pick Disease Type C Fibroblasts